Isolation and properties of nonspecific esterase of murine L 1210 leukemia cells.

Nonspecific esterases of mouse lymphatic leukemia cells L 1210 were isolated and partly purified by gel filtration or DEAE Sephadex ion exchange chromatography. Two forms of esterases of differing molecular weight were found. Both esterases have optimum pH 7.2 and are inhibited by serine esterase inhibitors as well as by thiol reagents. They are not influenced by chelating agent (EDTA).