抗诱变剂对λ噬菌体溶原性大肠杆菌中前噬菌体诱导的影响。

Effects of antimutagens on prophage induction in E coli lysogenic for lambda.

作者信息

Persons D L, Shankel D M

出版信息

Environ Mutagen. 1983;5(1):59-71. doi: 10.1002/em.2860050108.

PMID:6219871

Abstract

The effects of caffeine, acriflavine, spermine, quinacrine, and adenosine on spontaneous and UV-induced prophage induction in repair-proficient and -deficient strains of lysogenic Escherichia coli were examined. Caffeine caused a coinducing effect on UV induction in KMBL 160 (repair proficient) and KMBL 163 (uvrA), an anti-inducing effect in KMBL 161 (uvrE), and had no detectable effect in KMBL 164 (uvrC). Acriflavine increased UV induction in KMBL 160 and decreased it in all three repair-deficient mutant strains. Spontaneous induction was reduced by acriflavine in all four strains. Spermine caused a decrease in UV induction in KMBL 160 and KMBL 164, while it had limited effect in KMBL 161 and KMBL 163. For spontaneous induction spermine caused an increase in KMBL 160 and KMBL 163, a decrease in KMBL 164 and no detectable effect in KMBL 161. Quinacrine had no significant effect on spontaneous induction in KMBL 160 and KMBL 164, but caused a decrease in spontaneous induction in KMBL 161 and KMBL 163. Quinacrine produced no effect on UV induction in KMBL 160; however, it decreased UV induction in the three repair-deficient strains to some extent. A significant increase in spontaneous induction in KMBL 160 was caused by adenosine, while it had little effect in the repair-deficient strains. UV induction was not affected by adenosine.

摘要

研究了咖啡因、吖啶黄、精胺、喹吖因和腺苷对溶源性大肠杆菌修复 proficient 和 -deficient 菌株中自发和紫外线诱导的原噬菌体诱导的影响。咖啡因对 KMBL 160（修复 proficient）和 KMBL 163（uvrA）中的紫外线诱导有共诱导作用，对 KMBL 161（uvrE）有抗诱导作用，而在 KMBL 164（uvrC）中未检测到影响。吖啶黄增加了 KMBL 160 中的紫外线诱导，并在所有三种修复缺陷突变菌株中降低了它。在所有四种菌株中，吖啶黄都降低了自发诱导。精胺在 KMBL 160 和 KMBL 164 中导致紫外线诱导减少，而在 KMBL 161 和 KMBL 163 中作用有限。对于自发诱导，精胺在 KMBL 160 和 KMBL 163 中导致增加，在 KMBL 164 中导致减少，而在 KMBL 161 中未检测到影响。喹吖因对 KMBL 160 和 KMBL 164 中的自发诱导没有显著影响，但在 KMBL 161 和 KMBL 163 中导致自发诱导减少。喹吖因对 KMBL 160 中的紫外线诱导没有影响；然而，它在一定程度上降低了三种修复缺陷菌株中的紫外线诱导。腺苷导致 KMBL 160 中的自发诱导显著增加，而在修复缺陷菌株中作用很小。紫外线诱导不受腺苷影响。