免疫皮肤病学研究。IX. 有机溶剂和酶对角质层抗原反应性的影响。
Studies in immunodermatology. IX. Effect of organic solvents and enzymes on the reactivity of stratum corneum antigens.
作者信息
Kumar V, Rogozinski T, Beutner E H, Jablonska S
出版信息
Int Arch Allergy Appl Immunol. 1983;71(2):112-6.
Explants of normal skin fail to react in direct immunofluorescence tests with stratum corneum antibodies. However, upon stripping with cellophane tape, the horny layer of such explants react in tissue culture. Swabbing of skin explants with ether and chloroform converts stratum corneum antigen (SCAg) from a nonreactive to a reactive form. Treatment with methanol, acetone or phosphate-buffered saline failed to bring about such a conversion. Treatment of skin explants with hyaluronidase and phosphilipase A converst SCAg of at least some skin explants to a reactive form. Treatment with trypsin, chymotrypsin and plasmin abolished the reactivity of SCAg upon prolonged incubation. However, upon short incubation with plasmin, SCAg was converted to a reactive form.
正常皮肤外植体在角质层抗体的直接免疫荧光试验中无反应。然而，用玻璃纸胶带剥离后，此类外植体的角质层在组织培养中会产生反应。用乙醚和氯仿擦拭皮肤外植体可将角质层抗原（SCAg）从不反应形式转变为反应形式。用甲醇、丙酮或磷酸盐缓冲盐水处理未能实现这种转变。用透明质酸酶和磷脂酶A处理皮肤外植体可将至少一些皮肤外植体的SCAg转变为反应形式。用胰蛋白酶、糜蛋白酶和纤溶酶处理，长时间孵育后会消除SCAg的反应性。然而，与纤溶酶短时间孵育后，SCAg会转变为反应形式。
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