Goffin V, Letawe C, Piérard G E
Belgian SSTC Research Center 5596, Department of Dermatopathology, University of Liège, Belgium.
Dermatology. 1997;195(4):321-4. doi: 10.1159/000245980.
Organic solvents alter the stratum corneum structure and barrier function.
To measure the effect of various solvents upon human stratum corneum using the ex vivo corneoxenometry bioassay which is a variant of corneosurfametry.
Corneoxenometry entails collection of human stratum corneum by cyanoacrylate. The material is immersed in organic solvents for periods ranging from 1 to 120 min. After staining the samples with a toluidine blue-basic fuchsin solution, the color is measured using reflectance colorimetry. Solvent aggressivity to the stratum corneum correlates with the color darkening of the samples.
The least aggressive solvent was hexane, followed by ethanol, methanol, hexane-ethanol, chloroform, chloroform-methanol and hexane-methanol. The influence of contact time between solvents and the stratum corneum showed a logarithmic pattern which varied according to the solvent.
Data are in line with previous experiments conducted in vivo and in vitro, thus indicating the predictive value of corneoxenometry. Such a bioassay may avoid hazards of some in vivo human testings.
有机溶剂会改变角质层结构和屏障功能。
使用角质层透光度生物测定法（一种角质层表面张力测定法的变体）来测量各种溶剂对人体角质层的影响。
角质层透光度生物测定法需要用氰基丙烯酸酯收集人体角质层。将材料浸入有机溶剂中1至120分钟不等。在用甲苯胺蓝-碱性品红溶液对样品染色后，使用反射比色法测量颜色。溶剂对角质层的侵蚀性与样品颜色变深相关。
侵蚀性最小的溶剂是己烷，其次是乙醇、甲醇、己烷-乙醇、氯仿、氯仿-甲醇和己烷-甲醇。溶剂与角质层接触时间的影响呈现对数模式，且因溶剂而异。
数据与之前在体内和体外进行的实验一致，从而表明角质层透光度生物测定法的预测价值。这种生物测定法可以避免一些人体体内测试的风险。
