Sharefkin J B, Lather C, Smith M, Rich N M
J Biomed Mater Res. 1983 Mar;17(2):345-57. doi: 10.1002/jbm.820170211.
Canine vascular endothelium labeled with indium-111-oxine was used as a marker of cell attachment to vascular prosthetic surfaces with complex textures. Primarily cultured and freshly harvested endothelial cells both took up the label rapidly. An average of 72% of a 32 micro Ci labeling dose was taken up by 1.5 X 10(6) cells in 10 min in serum-free medium. Over 95% of freshly labeled cells were viable by trypan blue tests and only 5% of the label was released after 1 h incubations at 37 degrees C. Labeled and unlabeled cells had similar rates of attachment to plastic dishes. Scanning electron microscopic studies showed that labeled cells retained their ability to spread on tissue culture dishes even at low (1%) serum levels. Labeled endothelial cells seeded onto Dacron or expanded polytetrafluoroethylene vascular prostheses by methods used in current surgical models could be identified by autoradiography of microscopic sections of the prostheses, and the efficiency of cell attachment to the prosthesis could be measured by gamma counting. Indium-111 labeling affords a simple and rapid way to measure initial cell attachment to, and distribution on, vascular prosthetic materials. The method could also allow measurement of early cell loss from a flow surface in vivo by using external gamma imaging.
用铟 - 111 - 奥克辛标记的犬血管内皮细胞被用作细胞附着于具有复杂纹理的血管假体表面的标志物。原代培养和刚收获的内皮细胞都能迅速摄取该标记物。在无血清培养基中，1.5×10⁶个细胞在10分钟内平均摄取了32微居里标记剂量的72%。通过台盼蓝试验，超过95%的新标记细胞是活的，并且在37℃孵育1小时后，只有5%的标记物被释放。标记和未标记的细胞对塑料培养皿的附着率相似。扫描电子显微镜研究表明，即使在低（1%）血清水平下，标记细胞仍能保持在组织培养皿上铺展的能力。通过当前手术模型中使用的方法将标记的内皮细胞接种到涤纶或膨体聚四氟乙烯血管假体上，可以通过假体显微切片的放射自显影来识别，并且可以通过γ计数来测量细胞对假体的附着效率。铟 - 111标记提供了一种简单快速的方法来测量细胞对血管假体材料的初始附着及其分布。该方法还可以通过使用外部γ成像来测量体内流动表面早期的细胞损失。
