使用X射线吸收边光谱法进行铜（I）的定量测定：2型耗尽漆树漆酶中还原双核铜位点的氧化

Quantitative Cu(I) determination using X-ray absorption edge spectroscopy: oxidation of the reduced binuclear copper site in type 2 depleted Rhus laccase.

作者信息

Hahn J E, Co M S, Spira D J, Hodgson K O, Solomon E I

出版信息

Biochem Biophys Res Commun. 1983 Apr 29;112(2):737-45. doi: 10.1016/0006-291x(83)91524-3.

Abstract

We report a procedure, through difference comparison of X-ray absorption edge spectra, for the quantitative determination of Cu(I) content in copper complexes of mixed oxidation state composition. This technique is tested on copper model systems and then used to quantitatively determine that untreated T2D Rhus laccase contains 70 +/- 15% Cu(I). Whereas excess ferricyanide is demonstrated not to alter the Cu(I) content of the untreated T2D, aqueous peroxide and nitrite at pH 6.0 are shown to oxidize the cuprous type 3 site and generate met T2D protein forms.

摘要

我们报告了一种通过X射线吸收边谱的差异比较来定量测定混合氧化态组成的铜配合物中Cu(I)含量的方法。该技术在铜模型系统上进行了测试，然后用于定量测定未处理的T2D漆树漆酶含有70±15%的Cu(I)。结果表明，过量的铁氰化物不会改变未处理的T2D的Cu(I)含量，而在pH 6.0时，过氧化氢水溶液和亚硝酸盐会氧化亚铜型3位点并生成T2D蛋白的高铁形式。