用亚铁氰化物对漆树漆酶和2型铜缺失酶进行滴定。铜位点的相互关系。
Titrations with ferrocyanide of japanese-lacquer-tree (Rhus vernicifera) laccase and of the type 2 copper-depleted enzyme. Interrelation of the copper sites.
作者信息
Morpurgo L, Graziani M T, Desideri A, Rotilio G
出版信息
Biochem J. 1980 May 1;187(2):367-70. doi: 10.1042/bj1870367.
Abstract
- Redox titrations are reported of the metal centres in Japanese-lacquer-tree (Rhus vernicifera) laccase with ferrocyanide. 2. The redox potential of Type 1 Cu was found to increase with ferrocyanide concentration up to a limiting value similar to that for the Type 1 Cu in Type 2 Cu-depleted enzyme (which is independent of ferrocyanide concentration). 3. The redox potential of the two-electron acceptor (Type 3 Cu) is also independent of ferrocyanide concentration in Type 2 Cu-depleted enzyme and lower than values reported for the native enzyme. 4. The two-electron acceptor is present in the oxidized state in the Type 2 Cu-depleted enzyme, though the latter lacks the 330 nm absorption band. 5. The redox potential of Type 2 Cu also depends on ferrocyanide concentration, at least in the presence of azide. 6. The redox potentials are affected by freezing the solutions and/or addition of azide, the latter binding to Type 2 Cu with affinity dependent on the redox state of the two-electron acceptor.
摘要
- 报道了用亚铁氰化物对漆树漆酶中的金属中心进行氧化还原滴定。2. 发现1型铜的氧化还原电位随亚铁氰化物浓度增加而升高，直至达到一个极限值，该极限值与2型铜缺失酶中1型铜的极限值相似（该极限值与亚铁氰化物浓度无关）。3. 在2型铜缺失酶中，双电子受体（3型铜）的氧化还原电位也与亚铁氰化物浓度无关，且低于天然酶报道的值。4. 尽管2型铜缺失酶缺乏330nm吸收带，但双电子受体在该酶中以氧化态存在。5. 2型铜的氧化还原电位也取决于亚铁氰化物浓度，至少在有叠氮化物存在时如此。6. 氧化还原电位受溶液冷冻和/或叠氮化物添加的影响，叠氮化物与2型铜结合，其亲和力取决于双电子受体的氧化还原状态。
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本文引用的文献
1
Purification and physico-chemical properties of laccase.漆酶的纯化及其物理化学性质
Biochim Biophys Acta. 1958 Oct;30(1):44-52. doi: 10.1016/0006-3002(58)90239-7.
2
Optical properties of japanese-lacquer-tree (Rhus vernicifera) laccase depleted of type 2 copper(II). Involvement of type-2 copper(II) in the 330nm chromophore.去除2型铜（II）的日本漆树漆酶的光学性质。2型铜（II）在330nm发色团中的作用。
Biochem J. 1980 May 1;187(2):361-6. doi: 10.1042/bj1870361.
3
The electron-accepting sites in Rhus vernicifera laccase as studied by anaerobic oxidation-reduction titrations.通过厌氧氧化还原滴定法研究漆树漆酶中的电子接受位点。
Eur J Biochem. 1971 Feb;18(4):463-8. doi: 10.1111/j.1432-1033.1971.tb01264.x.
4
Oxidation-reduction potential of the ferro-ferricyanide system in buffer solutions.缓冲溶液中亚铁氰化铁体系的氧化还原电位。
Biochim Biophys Acta. 1973 Apr 5;292(3):509-15. doi: 10.1016/0005-2728(73)90001-7.
5
Oxidation-reduction potentials of the electron acceptors in laccases and stellacyanin.漆酶和紫铜氧化酶中电子受体的氧化还原电位。
Biochim Biophys Acta. 1972 Aug 17;275(2):245-59. doi: 10.1016/0005-2728(72)90045-x.
6
Mechanistic studies of the reduction of Rhus vernicifera laccase by hydroquinone.
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7
Electron transfer reactions of copper proteins.铜蛋白的电子转移反应。
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8
Stereochemistry of anion complexes of type 2 Cu(II) in Rhus vernicifera laccase. Analogy with superoxide dismutase and Cu(II) carbonic anhydrase.
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J Am Chem Soc. 1976 Feb 18;98(4):1029-31. doi: 10.1021/ja00420a035.
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