大肠杆菌中UDP-N-乙酰葡糖胺和UDP-N-乙酰葡糖胺-烯醇丙酮酸的总体水平及其与肽聚糖合成的相关性。
Pool levels of UDP N-acetylglucosamine and UDP N-acetylglucosamine-enolpyruvate in Escherichia coli and correlation with peptidoglycan synthesis.
作者信息
Mengin-Lecreulx D, Flouret B, van Heijenoort J
出版信息
J Bacteriol. 1983 Jun;154(3):1284-90. doi: 10.1128/jb.154.3.1284-1290.1983.
Abstract
A high-pressure liquid chromatography procedure was developed for the isolation and quantitation of UDP-N-acetylglucosamine, UDP-N-acetylglucosamine-enolpyruvate, and UDP-N-acetylmuramic acid, which are the early cytoplasmic precursors of bacterial peptidoglycan. In exponential-phase cells of Escherichia coli K-12, the intracellular concentration of UDP-N-acetylglucosamine was about 100 microM, whereas that of UDP-N-acetylglucosamine-enolpyruvate was only 2 microM. The phosphoenolpyruvate: UDP-N-acetylglucosamine transferase and UDP-N-acetylglucosamine-enolpyruvate reductase activities were investigated in extracts from E. coli. These activities appeared to be present in amounts sufficient for the ongoing rate of peptidoglycan synthesis. Certain uridine nucleotide peptidoglycan precursors were found to inhibit phosphoenolpyruvate: UDP-N-acetylglucosamine transferase activity.
摘要
开发了一种高压液相色谱法，用于分离和定量尿苷二磷酸-N-乙酰葡糖胺、尿苷二磷酸-N-乙酰葡糖胺-烯醇丙酮酸和尿苷二磷酸-N-乙酰胞壁酸，它们是细菌肽聚糖的早期细胞质前体。在大肠杆菌K-12的指数生长期细胞中，尿苷二磷酸-N-乙酰葡糖胺的细胞内浓度约为100微摩尔，而尿苷二磷酸-N-乙酰葡糖胺-烯醇丙酮酸的浓度仅为2微摩尔。对大肠杆菌提取物中的磷酸烯醇丙酮酸：尿苷二磷酸-N-乙酰葡糖胺转移酶和尿苷二磷酸-N-乙酰葡糖胺-烯醇丙酮酸还原酶活性进行了研究。这些活性的量似乎足以满足肽聚糖合成的当前速率。发现某些尿苷核苷酸肽聚糖前体可抑制磷酸烯醇丙酮酸：尿苷二磷酸-N-乙酰葡糖胺转移酶活性。
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Pool levels of UDP N-acetylglucosamine and UDP N-acetylglucosamine-enolpyruvate in Escherichia coli and correlation with peptidoglycan synthesis.大肠杆菌中UDP-N-乙酰葡糖胺和UDP-N-乙酰葡糖胺-烯醇丙酮酸的总体水平及其与肽聚糖合成的相关性。
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