Long P M, Sommer C V, Kong Y M
Cell Immunol. 1983 Jun;78(2):266-75. doi: 10.1016/0008-8749(83)90281-2.
Murine histocompatibility antigens were solubilized from the spleens and lungs of C57BL/6 (H-2b) animals with hypertonic salt (3 M KC1). Aggregate-free soluble antigens were incubated with nonadherent lymph node cells from BALB/c (H-2d) mice for 18 hr prior to their use as responder cells in the mixed-lymphocyte reaction (MLR). It was found that the generation of cytotoxic cells was suppressed while the proliferative response was not affected. The observed suppression was not due to a shift in the kinetics of the generation of cytotoxicity as determined throughout a 10-day culture period. The suppression was specific in that the response in MLR to unrelated H-2f stimulator cells and the subsequent generation of cytotoxic cells were unchanged. Using various H-2 recombinant strains as target cells in the assay of cell-mediated lympholysis, suppression of cytotoxicity was observed when the D end, but not the K end, was shared with the C57BL/6 strain from which the antigens were derived.
用高渗盐（3M KCl）从C57BL/6（H-2b）动物的脾脏和肺中溶解小鼠组织相容性抗原。在用作混合淋巴细胞反应（MLR）中的反应细胞之前，将无聚集的可溶性抗原与来自BALB/c（H-2d）小鼠的非贴壁淋巴结细胞孵育18小时。发现细胞毒性细胞的生成受到抑制，而增殖反应未受影响。在整个10天的培养期内确定，观察到的抑制并非由于细胞毒性生成动力学的改变。这种抑制是特异性的，因为在MLR中对不相关的H-2f刺激细胞的反应以及随后细胞毒性细胞的生成未发生变化。在细胞介导的淋巴细胞溶解试验中，使用各种H-2重组菌株作为靶细胞，当与抗原来源的C57BL/6菌株共享D端而非K端时，观察到细胞毒性受到抑制。
