低分子量淋巴因子成分对Fc和C3b受体介导的巨噬细胞功能的影响。

Effect of low-molecular-weight lymphokine components on the Fc and C3b receptor-mediated macrophage functions.

作者信息

Fóris G, Hauck M, Dezsö B, Medgyesi G A, Füst G

出版信息

Cell Immunol. 1983 Jun;78(2):276-84. doi: 10.1016/0008-8749(83)90282-4.

Abstract

Rat lymphokine (LK) components of 500--2500 MW separated on Sephadex G-15 column (FrA) were tested for their effect on Fc and C3b receptor activities of rat resident (rPM) and thioglycollate-provoked (pPM) peritoneal macrophages. Functions of the receptors were studied by measuring the adherence and uptake of 51Cr-labeled sheep red blood cells (SRBC) mediated by isolated rat anti-SRBC IgM or IgG2a antibodies and human C3, respectively. On rPMs mainly Fc mu receptors (Fc mu Rs) were affected by FrA; at low concentration (20 micrograms/ml) adherence was increased and phagocytosis was inhibited. At higher concentrations (40-80 micrograms/ml) a reverse effect was observed: adherence was inhibited and phagocytosis increased. On pPMs IgG2a-mediated functions were mainly affected by FrA with a concentration dependence like that observed with Fc mu Rs on rPM monolayers. A concentration-dependent enhancement of C3b receptor (C3bR)-mediated adherence by FrA was observed on both PM types. On pPms C3bR-mediated phagocytosis was enhanced as well.

摘要

在葡聚糖凝胶G - 15柱（组分A）上分离得到的分子量为500 - 2500的大鼠淋巴因子（LK）成分，检测其对大鼠驻留腹膜巨噬细胞（rPM）和巯基乙酸诱导的腹膜巨噬细胞（pPM）的Fc和C3b受体活性的影响。分别通过测量由分离的大鼠抗绵羊红细胞IgM或IgG2a抗体和人C3介导的51Cr标记的绵羊红细胞（SRBC）的黏附及摄取，来研究这些受体的功能。对于rPM，组分A主要影响Fcμ受体（FcμRs）；在低浓度（20微克/毫升）时，黏附增加而吞噬作用受到抑制。在较高浓度（40 - 80微克/毫升）时观察到相反的效果：黏附受到抑制而吞噬作用增强。对于pPM，IgG2a介导的功能主要受组分A影响，其浓度依赖性与在rPM单层上观察到的FcμRs相似。在两种类型的腹膜巨噬细胞上均观察到组分A对C3b受体（C3bR）介导的黏附有浓度依赖性增强作用。在pPM上，C3bR介导的吞噬作用也增强。