Mitsuma T, Nogimori T
Endocrinol Jpn. 1982 Dec;29(6):695-700. doi: 10.1507/endocrj1954.29.695.
The effects of streptozotocin-induced diabetes mellitus on the hypothalamic-pituitary-thyroid axis in rats were studied. Streptozotocin (60 mg/kg) was injected ip. Rats were decapitated at two and four weeks after the streptozotocin treatment. Thyrotropin releasing hormone (TRH), thyrotropin (TSH), thyroxine (T4), 3,3',5-triiodothyronine (T3), 3,3',5'-triiodothyronine (rT3), 3,3'-diiodothyronine (3,3'-T2) and 3',5'-diiodothyronine (3',5'-T2) were measured by means of the specific radioimmunoassay for each. Immunoreactive TRH (ir-TRH) contents in the hypothalamus significantly decreased at four weeks (p less than 0.02). Basal TSH levels in plasma significantly decreased (p less than 0.005, p less than 0.001), and plasma ir-TRH and TSH responses to cold were significantly inhibited after the streptozotocin treatment (p less than 0.001). The plasma TSH response to TRH was decreased, but not significantly. The plasma T4 and T3 levels fell significantly. RT3 did not change throughout the experiment. 3,3'-T2 levels in plasma fell significantly, whereas 3',5'-T2 increased. Blood glucose levels rose significantly after streptozotocin treatment, but insulin treatment led to partial restoration. The findings suggest that streptozotocin-induced diabetes mellitus affects various sites of the hypothalamic-pituitary-thyroid axis in rats.
研究了链脲佐菌素诱导的糖尿病对大鼠下丘脑 - 垂体 - 甲状腺轴的影响。腹腔注射链脲佐菌素（60mg/kg）。在链脲佐菌素治疗后两周和四周将大鼠断头。通过特异性放射免疫分析法分别测定促甲状腺激素释放激素（TRH）、促甲状腺激素（TSH）、甲状腺素（T4）、三碘甲状腺原氨酸（T3）、反三碘甲状腺原氨酸（rT3）、3,3'-二碘甲状腺原氨酸（3,3'-T2）和3',5'-二碘甲状腺原氨酸（3',5'-T2）。下丘脑免疫反应性TRH（ir-TRH）含量在四周时显著降低（p<0.02）。血浆基础TSH水平显著降低（p<0.005，p<0.001），链脲佐菌素治疗后血浆ir-TRH和TSH对寒冷的反应显著受到抑制（p<0.001）。血浆TSH对TRH的反应降低，但不显著。血浆T4和T3水平显著下降。整个实验过程中rT3没有变化。血浆中3,3'-T2水平显著下降，而3',5'-T2升高。链脲佐菌素治疗后血糖水平显著升高，但胰岛素治疗导致部分恢复。这些发现表明链脲佐菌素诱导的糖尿病会影响大鼠下丘脑 - 垂体 - 甲状腺轴的各个部位。
