Beaudet R, Frenette M, Portelance V
Ann Immunol (Paris). 1983 Mar-Apr;134C(2):215-26. doi: 10.1016/s0769-2625(83)80093-2.
The comparative anti-tumour activity of cell walls, deproteinized cell walls and cell wall skeleton (CWS) isolated from four BCG substrains (Canadian, Russian, Glaxo and Swedish) was determined against Ehrlich ascitic carcinoma in CF-1 mice and against L1210 lymphoid leukaemia in B6D2F1/J mice. In Ehrlich carcinoma, the protocol of injection was shown to be of critical importance, since high and low levels of protection and even facilitation of tumour growth were observed according to the protocol used. The oil emulsion used as the vehicle for injection of the fractions (control groups) induced facilitation of tumour growth with some protocols. The highest levels of protection were observed when treatment was performed on day -6 for a single i. p. injection or on days -14 and 0 for two injections. Using the former protocol of immunization, the highest level of protection was detected with cell walls and deproteinized cell walls. CWS isolated from the Canadian and Glaxo substrains were found to be inactive, whereas those isolated from the Swedish and Russian substrains induced significant levels of protection. In L1210 leukaemia, very few preparations induced significant protection. CWS preparations did not induce protection. Canadian and Russian deproteinized cell walls and Canadian cell walls (100 and 250 micrograms, respectively) induced some protection. The use of different protocols of injection did not increase the anti-tumour activity of the Canadian cell walls. The most active preparation in both tumour systems was that of Canadian deproteinized cell walls.
测定了从四种卡介苗菌株（加拿大株、俄罗斯株、葛兰素株和瑞典株）分离得到的细胞壁、脱蛋白细胞壁和细胞壁骨架（CWS）对CF-1小鼠艾氏腹水癌以及对B6D2F1/J小鼠L1210淋巴细胞白血病的比较抗肿瘤活性。在艾氏癌实验中，注射方案被证明至关重要，因为根据所采用的方案，观察到了高水平和低水平的保护作用，甚至肿瘤生长的促进作用。用作各组分注射载体的油乳剂在某些方案中会促进肿瘤生长。当在第-6天进行单次腹腔注射治疗或在第-14天和第0天进行两次注射时，观察到最高水平的保护作用。采用前一种免疫方案时，细胞壁和脱蛋白细胞壁检测到最高水平的保护作用。从加拿大株和葛兰素株分离得到的CWS无活性，而从瑞典株和俄罗斯株分离得到的CWS诱导了显著水平的保护作用。在L1210白血病实验中，很少有制剂诱导出显著的保护作用。CWS制剂未诱导出保护作用。加拿大和俄罗斯的脱蛋白细胞壁以及加拿大细胞壁（分别为100微克和250微克）诱导了一定的保护作用。采用不同的注射方案并未提高加拿大细胞壁的抗肿瘤活性。在两个肿瘤系统中最具活性的制剂是加拿大脱蛋白细胞壁制剂。
