Whiteside T L, Kumagai Y, Roumm A D, Almendinger R, Rodnan G P
Arthritis Rheum. 1983 Jul;26(7):841-7. doi: 10.1002/art.1780260704.
Three different in vitro assays--immunofluorescence with monoclonal anti-T cell reagents, enumeration of T gamma cells, and nonspecific suppressor cell function--were used for the analysis of suppressor lymphocytes in the circulation of 28 patients with progressive systemic sclerosis (PSS, scleroderma) and 20 normal individuals. Both OKT8+ and T gamma lymphocytes were significantly reduced (P less than 0.001 and P less than 0.02, respectively) in patients with PSS compared with controls. The OKT4/OKT8 ratio was increased (P less than 0.02). However, the mean suppressor cell index (SCI) of 1.9 (range 0.4-6.6) for patients with PSS was not significantly different (P greater than 0.05) from the SCI of 2.9 (range 1.2-14) for controls. Eleven of the patients had depressed suppressor cell function as indicated by the index value of less than 1.2. In only 5 of these patients, simultaneously measured T gamma and OKT8+ cells were reduced and OKT4+ lymphocytes were concomitantly increased. There were no significant correlations between the numbers of T gamma or OKT8+ cells and the SCI in patients and controls. Neither depressed suppressor cell function nor the OKT4+/OKT8+ ratio greater than 4.2 (greater than 2 SD of normal) in the patients could be related to other immunologic findings, to disease duration and severity, or to involvement of internal organs. These results suggest that depressed suppressor cell activity and immunoregulatory T cell imbalance in PSS may not be directly related to the pathogenesis of the disease.
采用三种不同的体外检测方法——用单克隆抗T细胞试剂进行免疫荧光检测、Tγ细胞计数以及非特异性抑制细胞功能检测——对28例进行性系统性硬化症（PSS，硬皮病）患者和20名正常个体循环中的抑制性淋巴细胞进行分析。与对照组相比，PSS患者的OKT8⁺和Tγ淋巴细胞均显著减少（分别为P<0.001和P<0.02）。OKT4/OKT8比值升高（P<0.02）。然而，PSS患者的平均抑制细胞指数（SCI）为1.9（范围0.4 - 6.6），与对照组SCI为2.9（范围1.2 - 14）相比，差异无统计学意义（P>0.05）。11例患者的抑制细胞功能降低，指数值小于1.2。在这些患者中，只有5例同时检测到Tγ和OKT8⁺细胞减少且OKT4⁺淋巴细胞同时增加。患者和对照组中Tγ或OKT8⁺细胞数量与SCI之间均无显著相关性。患者中抑制细胞功能降低以及OKT4⁺/OKT8⁺比值大于4.2（大于正常均值的2个标准差）均与其他免疫学发现、疾病持续时间和严重程度或内脏受累无关。这些结果表明，PSS中抑制细胞活性降低和免疫调节性T细胞失衡可能与疾病的发病机制无直接关系。
