May R D, Slavin S, Vitetta E S
J Immunol. 1983 Sep;131(3):1108-14.
Total lymphoid irradiation (TLI) is a highly effective modality for inducing immunosuppression and transplantation tolerance. The cellular basis for this immunosuppression is not clear, although T cells have been implicated. To study further the effect of TLI on the immune system, we have examined the B cells and suppressor cells in the spleens from TLI-conditioned mice. Our results indicate that after TLI, the spleen is rapidly repopulated with many large, immature cells. The probable source of these cells is the shielded bone marrow (BM). The B cells from TLI-conditioned mice are transiently immature and hyporesponsive in vitro to a T-independent antigen. Spleen cells from TLI-conditioned mice nonspecifically suppress the in vitro T-independent anti-TNP response of normal B cells. The suppressor cells lack both B and T cell markers and adhere to Sephadex G-10. The suppressor cells in spleens from TLI-treated mice bear a number of similarities to those present in normal BM. When normal BM cells were analyzed by indirect immunofluorescence for the presence of the Mac-1 antigen, two populations of suppressor cells could be identified: one was Mac-1+ and the other was Mac-1-. These data are consistent with the possibility that a subpopulation of the suppressor cells found in normal BM and in the spleens from TLI-conditioned mice are immature cells of the monocytic/granulocytic lineage.
全身淋巴照射（TLI）是诱导免疫抑制和移植耐受的一种高效方法。尽管T细胞与之有关，但这种免疫抑制的细胞基础尚不清楚。为了进一步研究TLI对免疫系统的影响，我们检测了经TLI处理的小鼠脾脏中的B细胞和抑制细胞。我们的结果表明，TLI后，脾脏迅速被许多大的未成熟细胞重新填充。这些细胞可能的来源是受保护的骨髓（BM）。经TLI处理的小鼠的B细胞在体外对非T细胞依赖性抗原暂时不成熟且反应低下。经TLI处理的小鼠的脾细胞非特异性抑制正常B细胞的体外非T细胞依赖性抗TNP反应。抑制细胞既缺乏B细胞标记也缺乏T细胞标记，并黏附于葡聚糖凝胶G-10。经TLI处理的小鼠脾脏中的抑制细胞与正常BM中的抑制细胞有许多相似之处。当通过间接免疫荧光分析正常BM细胞中Mac-1抗原的存在时，可以鉴定出两种抑制细胞群体：一种是Mac-1阳性，另一种是Mac-1阴性。这些数据与以下可能性一致，即在正常BM和经TLI处理的小鼠脾脏中发现的抑制细胞亚群是单核细胞/粒细胞系的未成熟细胞。
