Zan-Bar I
Eur J Immunol. 1983 Jan;13(1):35-40. doi: 10.1002/eji.1830130109.
The effect of fractionated total lymphoid irradiation (TLI) on the maturation of B cells was examined in mice. At various times after irradiation of the mice, their spleen cells were tested for the mitogenic responses to dextran sulfate and lipopolysaccharide. In parallel the cells were stained with fluorescent anti-Thy-1 and various anti-Ig antisera, and were analyzed on a fluorescence-activated cell sorter (FACS). By comparison with normal spleen cells the cells of TLI-treated mice gave a high response to dextran sulfate and a low response to lipopolysaccharide. FACS analysis revealed that TLI-treated spleen contains elevated numbers of B cells bearing high IgM and low IgD on the surface. The B cells of TLI-treated mice retained these immature characteristics even two to four months after the last irradiation. These findings indicate that TLI causes a longlasting blockage in B cell maturation processes.
在小鼠中检测了分次全身淋巴照射（TLI）对B细胞成熟的影响。在照射小鼠后的不同时间，检测其脾细胞对硫酸葡聚糖和脂多糖的促有丝分裂反应。同时，用荧光抗Thy-1和各种抗Ig抗血清对细胞进行染色，并在荧光激活细胞分选仪（FACS）上进行分析。与正常脾细胞相比，TLI处理小鼠的细胞对硫酸葡聚糖反应高，对脂多糖反应低。FACS分析显示，TLI处理的脾中表面带有高IgM和低IgD的B细胞数量增加。即使在最后一次照射后两到四个月，TLI处理小鼠的B细胞仍保留这些不成熟特征。这些发现表明，TLI导致B细胞成熟过程中出现长期阻滞。
