Further studies of the cytopathic effect in tissue cultures inoculated with CSF from patients with schizophrenia and other nervous system diseases.

Tissue cultures were inoculated with CSF from patients with schizophrenia and some other psychiatric and neurological illnesses. The CPE was readily seen in all 13 strains of human skin fibroblasts examined but could not be serially passaged. The results of retesting many specimens, either by the same worker or under full double blind conditions, usually agreed and thus indicate that the CPE was due to the CSF and not to non specific changes in the cultures. CPE was seen in cultures in which mycoplasmas could not be detected. Degenerated cultures show polymorphous nuclei with condensed nuclear chromatin and enlarged mitochondria with few cristae but virus particles were not identified. The physical properties of the agent in the CSF that induced the CPE were the same as those reported earlier for CSF from schizophrenic patients.