Buonassisi V, Colburn P
Biochim Biophys Acta. 1983 Oct 4;760(1):1-12. doi: 10.1016/0304-4165(83)90118-6.
We have determined, using polyacrylamide gel electrophoresis, that endothelial cell cultures derived from rabbit aorta synthesize a wide spectrum of heparan sulfate proteoglycans. The electrophoretically slower-moving heparan sulfate proteoglycans have been isolated from the endothelial cell culture medium. Antibodies to these proteoglycan species have been raised in the goat. The goat antiserum binds selectively the heparan sulfate proteoglycans that were used for the immunization and does not cross-react with the other (faster moving) species. Only a moderate level of cross-reactivity was observed with the heparan sulfate proteoglycans synthesized by another cell line (presumably smooth muscle cells) of vascular derivation. These results support the suggestion that structural differences in the heparan sulfate proteoglycans are responsible for certain differences in function between the various cell types of the vessel wall.
我们通过聚丙烯酰胺凝胶电泳确定，源自兔主动脉的内皮细胞培养物能合成多种硫酸乙酰肝素蛋白聚糖。电泳迁移较慢的硫酸乙酰肝素蛋白聚糖已从内皮细胞培养基中分离出来。针对这些蛋白聚糖种类的抗体已在山羊体内产生。山羊抗血清选择性地结合用于免疫的硫酸乙酰肝素蛋白聚糖，且不与其他（迁移较快的）种类发生交叉反应。在由血管来源的另一种细胞系（可能是平滑肌细胞）合成的硫酸乙酰肝素蛋白聚糖中，仅观察到中等程度的交叉反应。这些结果支持了以下观点，即硫酸乙酰肝素蛋白聚糖的结构差异是血管壁各种细胞类型之间某些功能差异的原因。
