Perussia B, Dayton E T, Lazarus R, Fanning V, Trinchieri G
J Exp Med. 1983 Oct 1;158(4):1092-113. doi: 10.1084/jem.158.4.1092.
We report here that FcR for human monomeric IgG1 can be induced on cells of myeloid origin cultured in the presence of IFN gamma for 8 h. Supernatant fluids from cultures of lymphocytes infected with a variety of viruses or cocultured with cell lines have the same FcR enhancing effect as IFN gamma. We identify the factor in the supernatant fluid responsible for the induction as immune interferon. Among the different types of IFN, only the gamma type (both purified and recombinant) specifically induces the appearance of FcR for monomeric IgG1 on normal and leukemic myeloid cells but not on cells of lymphoid origin. This effect is also evident on mature PMN. We show that the specificity and the affinity of the receptor induced on HL-60 promyelocytic cells, peripheral blood monocytes, and PMN are identical to those of the receptor spontaneously present on the same cells, except for PMN, which do not spontaneously express this type of receptor. The results of inhibition experiments performed with mouse IgG of and IgG3. These results suggest that the receptor present on human monocytes different isotypes indicate that the receptor can be inhibited by murine IgG2a or immature myeloid cells, selectively inducible by IFN gamma, has a specificity similar to the FcR1 described on mouse macrophages.
我们在此报告，在γ干扰素存在的情况下培养8小时，可在髓系来源的细胞上诱导出人单体IgG1的Fc受体。来自感染多种病毒的淋巴细胞培养物或与细胞系共培养的淋巴细胞培养物的上清液具有与γ干扰素相同的Fc受体增强作用。我们确定上清液中负责诱导的因子为免疫干扰素。在不同类型的干扰素中，只有γ型（纯化的和重组的）能特异性地诱导正常和白血病髓系细胞上出现单体IgG1的Fc受体，而在淋巴系来源的细胞上则不能。这种作用在成熟的PMN上也很明显。我们表明，在HL-60早幼粒细胞、外周血单核细胞和PMN上诱导的受体的特异性和亲和力与同一细胞上自发存在的受体相同，但PMN除外，PMN不会自发表达这种类型的受体。用小鼠IgG1和IgG3进行的抑制实验结果表明，存在于人类单核细胞上的受体可被小鼠IgG2a抑制，不同的同种型表明该受体可被小鼠IgG2a抑制，在未成熟髓系细胞上可被γ干扰素选择性诱导，其特异性与小鼠巨噬细胞上描述的FcR1相似。
