Sheela S, Pai S B
Appl Environ Microbiol. 1983 Aug;46(2):475-9. doi: 10.1128/aem.46.2.475-479.1983.
Fensulfothion (O,O-diethyl O-[4-(methylsulfinyl)phenyl]phosphorothioate), an organophosphorus pesticide used to control the golden nematode Heterodera rostochiensis, is used as a source of carbon by microorganisms isolated from soils treated with the pesticide. Two of the microbial isolates, Pseudomonas alcaligenes C1 and Alcaligenes sp. strain NC3, used more than 80% of the pesticide in 120 h in culture when supplemented as a source of carbon. P. alcaligenes C1, which showed maximal growth on fensulfothion, degraded the compound to p-methylsulfinyl phenol and diethyl phosphorothioic acid. The phenolic metabolite could be identified by conventional spectral analysis, whereas the spectral patterns of the phosphorus-containing metabolite suggested that the compound was complexed with some cellular molecules. However, utilization of the phosphoric acid ester and ethanol by P. alcaligenes C1 suggested that the microbe attacks fensulfothion by an initial hydrolysis of the compound and subsequent utilization of the phosphoric acid ester. The pathway of degradation of fensulfothion by P. alcaligenes is of great value in the detoxification of the pesticide residues and also in the environmentally stable phosphoric acid esters.
丰索磷（O,O-二乙基-O-[4-(甲基亚磺酰基)苯基]硫代磷酸酯）是一种用于防治金色线虫（罗氏异皮线虫）的有机磷农药，从用该农药处理过的土壤中分离出的微生物将其用作碳源。其中两株微生物分离株，即产碱假单胞菌C1和产碱菌属菌株NC3，在以丰索磷作为碳源补充时，在120小时的培养过程中消耗了超过80%的该农药。在丰索磷上生长最佳的产碱假单胞菌C1将该化合物降解为对甲基亚磺酰基苯酚和硫代磷酸二乙酯。酚类代谢产物可通过传统光谱分析鉴定，而含磷代谢产物的光谱模式表明该化合物与一些细胞分子络合。然而，产碱假单胞菌C1对磷酸酯和乙醇的利用表明，该微生物通过最初水解该化合物并随后利用磷酸酯来攻击丰索磷。产碱假单胞菌对丰索磷的降解途径在农药残留解毒以及环境稳定的磷酸酯方面具有重要价值。
