Rosenberg A, Alexander M
Appl Environ Microbiol. 1979 May;37(5):886-91. doi: 10.1128/aem.37.5.886-891.1979.
Bacteria able to utilize Aspon, Azodrin, Dasanit, diazinon, malathion, Orthene, parathion, Trithion, dimethoate, Dylox, methyl parathion, and Vapona as sole phosphorus sources were isolated from soil and sewage. Individual isolates used from 3 to 10 of these insecticides as sole phosphorus sources. The extent of growth of two Pseudomonas strains in media containing diazinon and malathion was in the range expected from the amount of insecticide supplied, and their proliferation resulted in disappearance of the chemical. Resting cells of the pseudomonads derived from cultures grown on diazinon or malathion but not orthophosphate caused extensive destruction of these two organophosphates in the presence or absence of chloramphenicol. Extracts of the two bacteria derived from organophosphate-grown cultures catalyzed the disappearance of Aspon, Azodrin, Dasanit, diazinon, malathion, Orthene, parathion, and Trithion but not dimethoate, Dylox, methyl parathion, and Vapona. Results from gas chromatographic analysis suggested that the extracts formed dimethyl phosphate from azodrin, dimethyl phosphorodithioate from malathion, diethyl phosphorodithioate from Trithion, and diethyl phosphorothioate from Dasanit, diazinon, and parathion. Dimethyl phosphate, dimethyl phosphorothioate , dimethyl phosphorodithioate, diethyl phosphate, and diethyl phosphorothioate were not used by the pseudomonads as sole phosphorus sources.
能够将阿斯普隆、谷硫磷、二溴磷、二嗪农、马拉硫磷、久效磷、对硫磷、三硫磷、乐果、敌百虫、甲基对硫磷和敌敌畏作为唯一磷源利用的细菌，是从土壤和污水中分离出来的。单个分离菌株将这些杀虫剂中的3至10种用作唯一磷源。两种假单胞菌菌株在含有二嗪农和马拉硫磷的培养基中的生长程度，处于根据所提供杀虫剂数量预期的范围内，并且它们的增殖导致了该化学品的消失。源自于在二嗪农或马拉硫磷而非正磷酸盐上生长的培养物的假单胞菌静止细胞，在有或没有氯霉素存在的情况下，都导致了这两种有机磷酸盐的大量破坏。源自于在有机磷酸盐上生长的培养物的两种细菌提取物，催化了阿斯普隆、谷硫磷、二溴磷、二嗪农、马拉硫磷、久效磷、对硫磷和三硫磷的消失，但没有催化乐果、敌百虫、甲基对硫磷和敌敌畏的消失。气相色谱分析结果表明，提取物从谷硫磷中形成了磷酸二甲酯，从马拉硫磷中形成了二甲基二硫代磷酸酯，从三硫磷中形成了二乙基二硫代磷酸酯，从二溴磷、二嗪农和对硫磷中形成了二乙基硫代磷酸酯。假单胞菌不将磷酸二甲酯、二甲基硫代磷酸酯、二甲基二硫代磷酸酯、二乙基磷酸酯和二乙基硫代磷酸酯用作唯一磷源。
