Ivashchenko A T, Uteulin K R, Li T
Biokhimiia. 1983 Aug;48(8):1256-60.
The effects of methanol, butanol, glycerol, glucose, sucrose and inorganic anions on the activity of soluble ATPase from mouse liver mitochondria were studied. Glycerol inhibited, while methanol stimulated the enzyme activity uncompetitively with respect to ATP and competitively with respect to each other. Glycerol-induced inhibition of ATPase was competitive with respect to sulphite; methanol competed with thiocyanate for the enzyme activity. The Arrhenius plots for ATPase revealed bends at 20 degrees and 30 degrees C in the presence of sulphite, chlorine, thiocyanate, glycerol and methanol. It was assumed that all the compounds tested influenced soluble ATPase by changing the nucleophilic activity of H2O.
研究了甲醇、丁醇、甘油、葡萄糖、蔗糖和无机阴离子对小鼠肝脏线粒体可溶性ATP酶活性的影响。甘油抑制该酶活性，而甲醇则以对ATP非竞争性、对彼此竞争性的方式刺激酶活性。甘油诱导的ATP酶抑制作用对亚硫酸盐具有竞争性；甲醇与硫氰酸盐竞争酶活性。在亚硫酸盐、氯、硫氰酸盐、甘油和甲醇存在的情况下，ATP酶的阿伦尼乌斯曲线在20℃和30℃处出现弯曲。据推测，所有测试的化合物都是通过改变H2O的亲核活性来影响可溶性ATP酶的。
