Scheiner O, Schulz T F, Schmid H U, Lambris J D, Alsenz J, Dierich M P
Immunobiology. 1983 Aug;165(2):211-24. doi: 10.1016/S0171-2985(83)80061-8.
The role of cell membrane-associated human factor H for the binding of cell-bound C3b to complement receptor-carrying (CR+) cells was investigated. Pretreatment of CR+ cells with antibodies to factor H inhibited the adherence of C3b-coated red cells to human tonsil lymphocytes (TL) and peripheral blood monocytes (M phi). The C3b receptor reactivity of human polymorphonuclear leucocytes (PMN) was not influenced and the one of Raji lymphoblastoid cells only slightly influenced; iC3b and C3d receptor reactivity was in no case affected. When diisopropylfluorophosphate (DFP) in a concentration of 0.1 mM was present during pretreatment of the CR+ cells with anti H, the antibodies gained the capacity to inhibit the adherence of C3b-coated erythrocytes to Raji cells; this effect was dose-dependent with respect to DFP. In contrast, there was no influence of DFP on the inhibition pattern of anti H in the case of TL and M phi. The adherence of C3b-coated erythrocytes to PMN remained unaffected by anti-H antibodies in the presence of DFP. Polyclonal as well as monoclonal antibodies directed against human factor I inhibited the binding of C3b cells to Raji cells but not to TL. Additionally, when anti I and anti H antibodies were both present, C3b receptor reactivity of Raji cells was inhibited to a larger extent than with either antibody alone; again, TL remained unaffected. Results obtained by washing the Raji cells before and after treatment with anti H and anti I suggest that the respective antibodies act on factor H primarily on the level of the cell membrane and on factor I in the fluid phase.
研究了细胞膜相关的人补体因子H在细胞结合的C3b与携带补体受体（CR+）细胞结合中的作用。用抗因子H抗体预处理CR+细胞可抑制C3b包被的红细胞与人扁桃体淋巴细胞（TL）和外周血单核细胞（M phi）的黏附。人多形核白细胞（PMN）的C3b受体反应性不受影响，Raji淋巴母细胞的反应性仅略有影响；iC3b和C3d受体反应性在任何情况下均未受影响。在用抗H抗体预处理CR+细胞期间，当存在浓度为0.1 mM的二异丙基氟磷酸酯（DFP）时，抗体获得了抑制C3b包被的红细胞与Raji细胞黏附的能力；这种效应在DFP方面呈剂量依赖性。相比之下，在TL和M phi的情况下，DFP对抗H的抑制模式没有影响。在DFP存在下，C3b包被的红细胞与PMN的黏附不受抗H抗体的影响。针对人补体因子I的多克隆抗体和单克隆抗体均抑制C3b细胞与Raji细胞的结合，但不抑制与TL的结合。此外，当抗I和抗H抗体同时存在时，Raji细胞的C3b受体反应性比单独使用任何一种抗体时受到更大程度的抑制；同样，TL不受影响。在用抗H和抗I处理前后洗涤Raji细胞所获得的结果表明，相应的抗体主要在细胞膜水平作用于因子H，在液相中作用于因子I。
