Orcutt-Thordarson N, Lum L G
Cell Immunol. 1983 Nov;82(1):184-95. doi: 10.1016/0008-8749(83)90152-1.
A new mitogenic system for in vitro immunoglobulin production induced by tetanus toxoid is presented and the role of T4 and T8 subsets in tetanus toxoid-induced in vitro immunoglobulin production is investigated. Purified T, T4, T8, and B cells from normal individuals previously immunized but not boosted with tetanus toxoid were cultured in helper and suppressor assays and the number of immunoglobulin-secreting cells were enumerated after culture using a hemolytic plaque assay. The regulatory roles of T4 and T8 cells in this tetanus toxoid system were compared with the role of these subsets after pokeweed mitogen stimulation. Although most of the immunoglobulin produced in the tetanus toxoid system was polyclonal, there were differences in the time course, the magnitude of the responses, the radiosensitivity of the subsets, and optimal T- to B-cell ratios for immunoglobulin production which distinguish the tetanus toxoid and pokeweed mitogen systems.
本文介绍了一种由破伤风类毒素诱导的用于体外免疫球蛋白产生的新的促有丝分裂系统，并研究了T4和T8亚群在破伤风类毒素诱导的体外免疫球蛋白产生中的作用。从先前接种过破伤风类毒素但未加强免疫的正常个体中纯化出T细胞、T4细胞、T8细胞和B细胞，在辅助和抑制试验中进行培养，并在培养后使用溶血空斑试验对免疫球蛋白分泌细胞的数量进行计数。将T4和T8细胞在该破伤风类毒素系统中的调节作用与这些亚群在商陆有丝分裂原刺激后的作用进行了比较。虽然在破伤风类毒素系统中产生的大多数免疫球蛋白是多克隆的，但在时间进程、反应强度、亚群的放射敏感性以及免疫球蛋白产生的最佳T细胞与B细胞比例方面存在差异，这些差异区分了破伤风类毒素和商陆有丝分裂原系统。
