Benedict W F, Khwaja T A
Med Pediatr Oncol. 1976;2(3):271-8. doi: 10.1002/mpo.2950020308.
A hamster fetal cell clone has been developed for in vitro chemotherapeutic studies as well as in an in vivo fibrosarcoma model system. Highly reproducible quantitative in vitro chemotherapeutic data can be obtained with this cell line within 5 days, and as few as 10(2) cells produce rapidly growing fibrosarcomas when injected subcutaneously into adult hamsters. We found using these cells in vitro that 1-beta-D-arabinofuranosylcytosine (ara-C) can antagonize the effect of 5-azacytidine (aza-C) if given simultaneously or if aza-C treatment is preceded by a 2-hr exposure to ara-c. Using the same cell line as in vivo model for chemotherapy it was also shown that ara-C and cyclocytidine significantly inhibited tumor growth. This hamster cell line may be quite useful as an in vitro/in vivo model system for the study of cancer chemotherapeutic agents.
已开发出一种仓鼠胎儿细胞克隆，用于体外化疗研究以及体内纤维肉瘤模型系统。使用该细胞系，可在5天内获得高度可重复的定量体外化疗数据，将低至10(2)个细胞皮下注射到成年仓鼠体内时，能产生快速生长的纤维肉瘤。我们在体外使用这些细胞发现，如果同时给予1-β-D-阿拉伯呋喃糖基胞嘧啶（ara-C）或在给予5-氮杂胞苷（aza-C）之前先让细胞接触ara-C 2小时，ara-C可拮抗aza-C的作用。使用同一细胞系作为体内化疗模型还表明，ara-C和环胞苷可显著抑制肿瘤生长。这种仓鼠细胞系作为研究癌症化疗药物的体外/体内模型系统可能非常有用。
