Stahl F W, Stahl M M, Young L, Kobayashi I
Genetics. 1982 Dec;102(4):599-613. doi: 10.1093/genetics/102.4.599.
Chi promotes Rec-mediated recombination between phage lambda DNA and the homologous plasmid lambda dv. In the absence of Chi, some of the interactions splice lambda dv into lambda, whereas others patch information from lambda dv into lambda. When Chi is in the phage DNA, splices and patches are increased in frequency by the same factor. This result strengthens the analogy between Chi and recombination-promoting elements in fungi. It also rules out one model for the previously reported orientation dependence of Chi phenotype.
Chi促进噬菌体λ DNA与同源质粒λ dv之间由Rec介导的重组。在没有Chi的情况下,一些相互作用将λ dv剪接到λ中,而其他相互作用则将λ dv的信息整合到λ中。当Chi存在于噬菌体DNA中时,剪接和整合的频率以相同的倍数增加。这一结果加强了Chi与真菌中重组促进元件之间的相似性。它也排除了之前报道的Chi表型方向依赖性的一种模型。