内切核酸酶在远处位点的作用激活重组酶Chi。
Activation of Chi, a recombinator, by the action of an endonuclease at a distant site.
作者信息
Stahl M M, Kobayashi I, Stahl F W, Huntington S K
出版信息
Proc Natl Acad Sci U S A. 1983 Apr;80(8):2310-3. doi: 10.1073/pnas.80.8.2310.
Abstract
Chi is an Escherichia coli recombinator specific to the recBC pathway. In phage lambda, its activity is dependent on its orientation with respect to the orientation of the chromosome packaging origin, cos [Kobayashi, I., Murialdo, H., Crasemann, J.M., Stahl, M. M. & Stahl, F. W. (1982) Proc. Natl. Acad. Sci. USA 79, 5981-5985]. Chi in an inactive state is activated by the in vivo action of a restriction endonuclease at a distant site. This result supports a model for Chi activation in which a recombination machine enters a DNA duplex at an end and travels until it encounters a Chi properly oriented with respect to the direction of enzyme movement. Events are then initiated that lead to exchange in the neighborhood of Chi.
摘要
Chi是一种特异性作用于recBC途径的大肠杆菌重组酶。在λ噬菌体中，其活性取决于它相对于染色体包装起始位点cos的方向[小林，I.，穆里亚尔多，H.，克拉泽曼，J.M.，斯塔尔，M.M.和斯塔尔，F.W.（1982年）《美国国家科学院院刊》79，5981 - 5985]。处于无活性状态的Chi会被远处位点的限制性内切核酸酶的体内作用激活。这一结果支持了一种Chi激活模型，即重组机器从一端进入DNA双链并移动，直到遇到相对于酶移动方向正确定向的Chi。然后引发一系列事件，导致在Chi附近发生交换。
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Separate sites for binding and nicking of bacteriophage lambda DNA by terminase.噬菌体λ DNA 由末端酶进行结合和切口的不同位点。
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Bacteriophage lambda DNA packaging: scanning for the terminal cohesive end site during packaging.噬菌体λDNA包装：包装过程中对末端粘性末端位点的扫描。
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Genetics. 1980 Sep;96(1):43-57. doi: 10.1093/genetics/96.1.43.
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