Kleinman H K, McGarvey M L, Hassell J R, Martin G R
Biochemistry. 1983 Oct 11;22(21):4969-74. doi: 10.1021/bi00290a014.
Basement membrane macromolecules, including type IV collagen, laminin, and heparan sulfate proteoglycan, do not aggregate when incubated alone. Rather, precipitation occurs in the presence of equimolar amounts of laminin and type IV collagen but variable amounts of heparan sulfate proteoglycan. This interaction requires native laminin and type IV collagen. Heparan sulfate proteoglycan increases the precipitation of laminin particularly in the presence of type IV collagen. Fibronectin does not cause type IV collagen to precipitate. These studies show that the components of basement membrane interact in a highly specific manner and suggest that such interactions may be involved in the deposition of basement membrane in situ.
基底膜大分子，包括IV型胶原、层粘连蛋白和硫酸乙酰肝素蛋白聚糖，单独孵育时不会聚集。相反，当存在等摩尔量的层粘连蛋白和IV型胶原以及不同量的硫酸乙酰肝素蛋白聚糖时会发生沉淀。这种相互作用需要天然的层粘连蛋白和IV型胶原。硫酸乙酰肝素蛋白聚糖会增加层粘连蛋白的沉淀，尤其是在IV型胶原存在的情况下。纤连蛋白不会导致IV型胶原沉淀。这些研究表明基底膜的成分以高度特异性的方式相互作用，并表明这种相互作用可能参与了基底膜在原位的沉积。
