Faure M, Frappaz A, Schmitt D, Dezutter-Dambuyant C, Thivolet J
Cell Immunol. 1984 Feb;83(2):271-9. doi: 10.1016/0008-8749(84)90306-x.
Human epidermal cells (EC) act as stimulator cells in the mixed-skin cell lymphocyte culture reaction (MSLR). To analyze the role of human epidermal Langerhans cells (LC) and indeterminate cells (IC), which are the only cells expressing the DR-Ia-like antigens in normal epidermis, in the generation of alloreactive cytotoxic T lymphocytes (CTL) in cell-mediated cytolysis, 18-hr 51Cr-release assays against PBL targets (targets autologous to stimulator EC) were conducted after allogeneic human MSLR. MSLR and CTL assays were conducted with, as stimulator cells, suspensions of normal human EC as controls, and EC after: (1) preincubation with anti-HLA-DR or OKT6 (specific for LC in EC suspension) monoclonal antibodies; (2) panning, a monolayer technique used to deplete EC suspensions in OKT6 or DR-positive cells. The generation of alloreactive CTL was found to occur only after allogeneic MSLR and when targets and stimulator cells were from the same donor; it was reduced by EC incubation: cytotoxic activity 26.66 +/- 3.84 (controls); 8.8 +/- 3.6 and 7.7 +/- 3.7 (EC incubated with OKT6 or anti-DR, respectively); it was reduced or abolished when the EC used in MSLR were depleted in OKT6 or DR-positive cells by panning. These findings demonstrate that human LC and IC are necessary for an optimal in vitro sensitization in MSLR and the subsequent in vitro generation of alloreactive CTL in man.
人表皮细胞（EC）在混合皮肤细胞淋巴细胞培养反应（MSLR）中作为刺激细胞。为了分析人表皮朗格汉斯细胞（LC）和不定形细胞（IC）在细胞介导的细胞溶解中同种异体反应性细胞毒性T淋巴细胞（CTL）产生中的作用，这两种细胞是正常表皮中唯一表达DR-Ia样抗原的细胞，在同种异体人MSLR后，针对PBL靶细胞（与刺激EC自体的靶细胞）进行了18小时的51Cr释放试验。使用正常人EC悬浮液作为对照以及经过以下处理后的EC作为刺激细胞进行MSLR和CTL试验：（1）与抗HLA-DR或OKT6（对EC悬浮液中的LC特异）单克隆抗体预温育；（2）淘选，一种用于耗尽OKT6或DR阳性细胞的EC悬浮液的单层技术。发现同种异体反应性CTL仅在同种异体MSLR后以及靶细胞和刺激细胞来自同一供体时产生；EC温育会使其减少：细胞毒性活性26.66±3.84（对照）；8.8±3.6和7.7±3.7（分别与OKT6或抗DR温育的EC）；当通过淘选使MSLR中使用的EC耗尽OKT6或DR阳性细胞时，其减少或消除。这些发现表明，人LC和IC对于MSLR中最佳的体外致敏以及随后人同种异体反应性CTL的体外产生是必需的。
