Faure M, Frappaz A, Schmitt D, Dezutter-Dambuyant C
Pathol Biol (Paris). 1984 Apr;32(4):233-8.
The ability of human normal skin epidermal cells (EC) to induce the generation of alloreactive cytotoxic T lymphocytes (CTL) was investigated in vitro using the Mixed Skin Cell lymphocyte Reaction (MSLR) model. In human MSLR, EC stimulated the proliferation of allogeneic peripheral blood lymphocytes (L) as measured, after 6 days, by 3H-thymidine uptake. In parallel, the generation of alloreactive CTL was tested in 18 hr 51CR release assays against L targets (targets autologous to EC that stimulated in MSLR). Allogeneic, not autologous MSLR, lead to the generation of CTL; alloreactive CTL were not generated against targets allogeneic to stimulating EC; no CTL activity occurred without previous stimulation by EC. These data indicate that in vitro MSLR may provide an useful tool for the investigation of lympho-epidermal interactions in man and our understanding of lymphocytotoxicity mechanisms that occur in vivo in response and/or directed to epidermal constituents .
使用混合皮肤细胞淋巴细胞反应（MSLR）模型在体外研究了人正常皮肤表皮细胞（EC）诱导同种异体反应性细胞毒性T淋巴细胞（CTL）生成的能力。在人MSLR中，EC刺激同种异体外周血淋巴细胞（L）增殖，6天后通过3H-胸腺嘧啶核苷摄取进行测量。同时，在针对L靶细胞（与在MSLR中刺激的EC自体的靶细胞）的18小时51Cr释放试验中测试同种异体反应性CTL的生成。同种异体而非自体MSLR导致CTL生成；未针对刺激EC的同种异体靶细胞生成同种异体反应性CTL；没有EC预先刺激则不发生CTL活性。这些数据表明，体外MSLR可能为研究人类淋巴-表皮相互作用以及我们对体内针对表皮成分产生反应和/或定向的淋巴细胞毒性机制的理解提供有用的工具。
