免疫球蛋白E（IgE）的小分子寡聚体导致大鼠嗜碱性白血病细胞表面的IgE受体大规模聚集。
Small oligomers of immunoglobulin E (IgE) cause large-scale clustering of IgE receptors on the surface of rat basophilic leukemia cells.
作者信息
Menon A K, Holowka D, Baird B
出版信息
J Cell Biol. 1984 Feb;98(2):577-83. doi: 10.1083/jcb.98.2.577.
Abstract
We examined the distribution of small oligomers of IgE bound to rat basophilic leukemia cells using fluorescence microscopy. The oligomers were seen to cluster into visible patches on the cell surface at 4 degrees C; at higher temperatures internalization also was observed. In contrast, cells labeled with IgE monomers remained predominantly ring-stained. Evidence is provided that the observed clustering of IgE oligomers is a cell-induced phenomenon, and the possible significance of this clustering is discussed in the context of the oligomer-triggered degranulation of rat basophilic leukemia cells.
摘要
我们使用荧光显微镜检查了与大鼠嗜碱性白血病细胞结合的IgE小寡聚物的分布情况。在4摄氏度时，可见寡聚物在细胞表面聚集成可见的斑块；在较高温度下，还观察到内化现象。相比之下，用IgE单体标记的细胞主要保持环状染色。有证据表明，观察到的IgE寡聚物聚集是一种细胞诱导现象，并在寡聚物触发大鼠嗜碱性白血病细胞脱颗粒的背景下讨论了这种聚集的可能意义。
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本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
The magnitude of signal amplification by ligand-induced receptor clustering.配体诱导的受体聚集导致的信号放大程度。
Nature. 1981 Jan 22;289(5795):322-3. doi: 10.1038/289322a0.
3
Larger oligomers of IgE are more effective than dimers in stimulating rat basophilic leukemia cells.在刺激大鼠嗜碱性白血病细胞方面，更大的IgE寡聚体比二聚体更有效。
J Immunol. 1980 Aug;125(2):701-10.
4
Monoclonal dinitrophenyl-specific murine IgE antibody: preparation, isolation, and characterization.单克隆二硝基苯基特异性小鼠IgE抗体：制备、分离及特性鉴定
J Immunol. 1980 Jun;124(6):2728-37.
5
Mechanism of binding of multivalent immune complexes to Fc receptors. 1. Equilibrium binding.多价免疫复合物与Fc受体的结合机制。1. 平衡结合。
Biochemistry. 1981 Oct 27;20(22):6326-34. doi: 10.1021/bi00525a007.
6
Enhanced molecular diffusibility in muscle membrane blebs: release of lateral constraints.肌膜小泡中分子扩散性增强：侧向限制的解除。
J Cell Biol. 1982 Jan;92(1):207-12. doi: 10.1083/jcb.92.1.207.
7
Non-coated membrane invaginations are involved in binding and internalization of cholera and tetanus toxins.无包被膜内陷参与霍乱毒素和破伤风毒素的结合与内化过程。
Nature. 1982 Apr 15;296(5858):651-3. doi: 10.1038/296651a0.
8
The antibody-induced clustering and endocytosis of HLA antigens on cultured human fibroblasts.抗体诱导培养的人成纤维细胞上HLA抗原的聚集和内吞作用。
Cell. 1980 Sep;21(2):429-38. doi: 10.1016/0092-8674(80)90479-1.
9
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