在新生期耐受小鼠中发现的细胞毒性T细胞前体。
Cytotoxic T-cell precursors revealed in neonatally tolerant mice.
作者信息
Stockinger B
出版信息
Proc Natl Acad Sci U S A. 1984 Jan;81(1):220-3. doi: 10.1073/pnas.81.1.220.
Abstract
Induction of neonatal tolerance leads to a profound reduction in cytotoxic T-lymphocyte precursor frequencies against the tolerated alloantigen ("tolerogen") as evaluated by limiting-dilution analysis. The curves obtained were linear within the range tested and, thus, did not yield evidence for any dissociation of cytotoxic precursors from regulatory cell populations. However, it could be shown that cytotoxic T-lymphocyte precursor frequencies against the tolerogen increased drastically if the tolerant spleen cells were adsorbed, prior to limiting-dilution culture, on monolayers of syngeneic blasts expressing receptors for the tolerogen but not if they were adsorbed on syngeneic blasts against third-party antigens. This finding implies that cytotoxic precursor cells against the tolerogen are not clonally deleted in tolerant animals but rather are suppressed by a regulatory cell population that is present at high frequency and presumably acts in an anti-idiotypic fashion.
摘要
通过有限稀释分析评估，诱导新生期耐受会导致针对耐受同种异体抗原（“耐受原”）的细胞毒性T淋巴细胞前体频率大幅降低。在所测试的范围内获得的曲线呈线性，因此，没有产生细胞毒性前体与调节细胞群体解离的证据。然而，可以证明，如果在有限稀释培养之前，将耐受的脾细胞吸附在表达耐受原受体的同基因胚细胞单层上，那么针对耐受原的细胞毒性T淋巴细胞前体频率会急剧增加，但如果将其吸附在针对第三方抗原的同基因胚细胞上则不会。这一发现意味着，在耐受动物中，针对耐受原的细胞毒性前体细胞并非克隆性缺失，而是被高频存在的调节细胞群体所抑制，推测该调节细胞群体以抗独特型方式发挥作用。
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Cytotoxic T-cell precursors revealed in neonatally tolerant mice.在新生期耐受小鼠中发现的细胞毒性T细胞前体。
Proc Natl Acad Sci U S A. 1984 Jan;81(1):220-3. doi: 10.1073/pnas.81.1.220.
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本文引用的文献
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Actively acquired tolerance of foreign cells.对外源细胞的主动获得性耐受。
Nature. 1953 Oct 3;172(4379):603-6. doi: 10.1038/172603a0.
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Limiting dilution assays for the determination of immunocompetent cell frequencies. I. Data analysis.用于确定免疫活性细胞频率的有限稀释分析。I. 数据分析。
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Recognition of polymorphic H-2 domains by T lymphocytes. I. Functional role of different H-2 domains for the generation of alloreactive cytotoxic T lymphocytes and determination of precursor frequencies.T淋巴细胞对多态性H-2结构域的识别。I. 不同H-2结构域在同种异体反应性细胞毒性T淋巴细胞生成中的功能作用及前体细胞频率的测定。
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Induced tolerance in F1 rats to anti-major histocompatibility complex receptors on parental T cells. Implications for self tolerance.诱导F1大鼠对亲代T细胞上抗主要组织相容性复合体受体产生耐受性。对自身耐受性的影响。
J Exp Med. 1981 Jun 1;153(6):1660-5. doi: 10.1084/jem.153.6.1660.
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Quantitative studies on T cell diversity. II. Determination of the frequencies and Lyt phenotypes of two types of precursor cells for alloreactive cytotoxic T cells in polyclonally and specifically activated splenic T cells.T细胞多样性的定量研究。II. 多克隆和特异性激活的脾T细胞中同种异体反应性细胞毒性T细胞两种前体细胞的频率及Lyt表型的测定
J Exp Med. 1981 Apr 1;153(4):857-70. doi: 10.1084/jem.153.4.857.
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Functional clonal deletion of cytotoxic T-lymphocyte precursors in chimeric thymus produced in vitro from embryonic Anlagen.在由胚胎原基体外产生的嵌合胸腺中，细胞毒性T淋巴细胞前体的功能性克隆缺失。
Proc Natl Acad Sci U S A. 1983 May;80(10):3045-9. doi: 10.1073/pnas.80.10.3045.
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Functional clonal deletion in immunological tolerance to major histocompatibility complex antigens.主要组织相容性复合体抗原免疫耐受中的功能性克隆清除
Proc Natl Acad Sci U S A. 1981 Jun;78(6):3844-7. doi: 10.1073/pnas.78.6.3844.
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Quantitation and cloning of cytolytic T lymphocytes and their precursors.细胞溶解性T淋巴细胞及其前体的定量与克隆
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