蛋白酶使瘙痒病病原体失活。
Inactivation of the scrapie agent by pronase.
作者信息
Cho H J
出版信息
Can J Comp Med. 1983 Oct;47(4):494-6.
Abstract
Scrapie agent derived from infected mouse brain was partially purified by agarose-polyacrylamide gel electrophoresis. The preparation was subjected to treatment with RNase A, DNase I, pronase, sodium dodecyl sulfate and sodium dodecyl sulfate-pronase combination. Almost total inactivation of scrapie infectivity resulted when the partially purified scrapie agent was treated with sodium dodecyl sulfate-pronase combination whereas the treatment with sodium dodecyl sulfate or pronase alone reduced the infectivity by about 98%. No significant reduction of scrapie infectivity was observed with either RNase A or DNase I treatments. These results confirm that the transmission of scrapie cannot be achieved in the absence of a protein component.
摘要
从感染小鼠脑中提取的瘙痒病病原体通过琼脂糖-聚丙烯酰胺凝胶电泳进行部分纯化。将该制剂用核糖核酸酶A、脱氧核糖核酸酶I、链霉蛋白酶、十二烷基硫酸钠以及十二烷基硫酸钠-链霉蛋白酶组合进行处理。当用十二烷基硫酸钠-链霉蛋白酶组合处理部分纯化的瘙痒病病原体时，瘙痒病感染力几乎完全丧失，而单独用十二烷基硫酸钠或链霉蛋白酶处理则使感染力降低约98%。用核糖核酸酶A或脱氧核糖核酸酶I处理均未观察到瘙痒病感染力有显著降低。这些结果证实，在没有蛋白质成分的情况下，瘙痒病无法传播。
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本文引用的文献
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Novel proteinaceous infectious particles cause scrapie.新型蛋白质感染性颗粒可引发羊瘙痒病。
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