Nemirovsky M S
Am J Reprod Immunol (1980). 1984 Jan-Feb;5(1):5-8. doi: 10.1111/j.1600-0897.1984.tb00279.x.
The involvement of antigens coded by the major histocompatibility complex in alloimmunization of guinea pigs with sperm has not been fully explored. Some of the "in vitro" correlates of cell-mediated immunity, such as the lymphocyte transformation test in presence of sperm cells, could be influenced, at least partially, by histoincompatibility. Outbred Hartley and inbred strain 13 guinea pigs were alloimmunized with sperm cells emulsified in Freund's complete adjuvant. The degree of histocompatibility between recipients and sperm cell donors was determined by using mixed lymphocyte culture assays. Stimulatory indices higher than four, indicating histoincompatibility, were obtained frequently between members of Hartley strain or between Hartley and strain 13 guinea pigs, but never between strain 13 animals. Sperm cells from histoincompatible guinea pigs failed to stimulate blastogenesis in lymphoid cells from nonimmunized animals. The lymphoblastogenic potential of allo- and isospermatozoa were compared. Lymphoid cells from Hartley and strain 13 guinea pigs alloimmunized with sperm cells were incubated in vitro with histocompatible and histoincompatible sperm cells. Both strain 13 and Hartley sperm cells gave a similar stimulatory index with lymphoid cells belonging to outbred or inbred alloimmunized guinea pigs. The use of fresh spermatozoa was of paramount importance. Frozen specimens lost their stimulatory capacity almost completely. Within the limits of the technique utilized here, we have failed to detect any influence of the antigens coded by the major histocompatibility complex of the guinea pig upon the sperm-induced lymphocyte transformation. These observations point to the sperm-specific autoantigens as being responsible for the lymphocyte proliferation.
主要组织相容性复合体编码的抗原在豚鼠精子同种免疫中的作用尚未得到充分研究。细胞介导免疫的一些“体外”相关指标，如在存在精子细胞时的淋巴细胞转化试验，可能至少部分地受到组织不相容性的影响。将远交系Hartley豚鼠和近交系13豚鼠用弗氏完全佐剂乳化的精子细胞进行同种免疫。通过混合淋巴细胞培养试验确定受体和精子细胞供体之间的组织相容性程度。在Hartley品系成员之间或Hartley和13品系豚鼠之间经常获得高于4的刺激指数，表明存在组织不相容性，但在13品系动物之间从未出现这种情况。来自组织不相容豚鼠的精子细胞未能刺激未免疫动物淋巴细胞的胚细胞生成。比较了同种精子和自身精子的淋巴细胞生成潜力。将用精子细胞进行同种免疫的Hartley和13品系豚鼠的淋巴细胞与组织相容和不相容的精子细胞在体外孵育。13品系和Hartley品系的精子细胞对远交或近交同种免疫豚鼠的淋巴细胞产生的刺激指数相似。使用新鲜精子至关重要。冷冻标本几乎完全丧失了刺激能力。在本文所采用技术的范围内，我们未能检测到豚鼠主要组织相容性复合体编码的抗原对精子诱导的淋巴细胞转化有任何影响。这些观察结果表明精子特异性自身抗原是淋巴细胞增殖的原因。
