变应性无精子生成与输精管结扎所致无精子生成的比较。II. 人和豚鼠输精管结扎后对精子细胞介导免疫的体外研究。
Comparison of allergic aspermatogenesis with that induced by vasectomy. II. In vitro studies of cell-mediated immunity to sperm after vasectomy in man and guinea-pig.
作者信息
Muir V Y, Turk J L, Hanley H G
出版信息
Clin Exp Immunol. 1977 Jun;28(3):461-6.
Transformation of peripheral blood leucocytes was shown to be a valid assay for cell-mediated immunity to sperm in male guinea-pigs immunized with homologous epididymal sperm (ES) in FCA. A heat-treated extract of ES (BES) was used for culture. Possible developement of cell-mediated immunity to sperm after vasectomy was investigated in patients and guinea-pigs, by culture of blood leucocytes before and at intervals after operation. Patients' leucocytes were cultured with a heat-treated extract of human seminal sperm (BHS); guinea-pigs' leucocytes were cultured with BES. These cultures showed no evidence of specific stimulation of leucocytes by sperm extract, up to 1 year after vasectomy in patients, or up to 6 months in guinea-pigs. Similarly, no evidence of delayed hypersensitivity could be demonstrated by skin tests with BES in animals vasectomized 1 year previously. We consider that this study establishes that cell-mediated immunity to sperm does not develop in either man or guinea-pigs, up to 1 year after conventional vasectomy.
在以弗氏完全佐剂（FCA）中的同源附睾精子（ES）免疫的雄性豚鼠中，外周血白细胞转化被证明是一种检测对精子细胞介导免疫的有效方法。使用经热处理的ES提取物（BES）进行培养。通过在输精管结扎术前及术后定期培养血白细胞，研究了输精管结扎术后患者和豚鼠对精子细胞介导免疫的可能发展情况。患者的白细胞用人精液精子的热处理提取物（BHS）培养；豚鼠的白细胞用BES培养。这些培养物显示，在患者输精管结扎后长达1年或豚鼠长达6个月的时间里，没有证据表明精子提取物对白细胞有特异性刺激。同样，在1年前接受输精管结扎的动物中，用BES进行皮肤试验也未显示出迟发型超敏反应的证据。我们认为，这项研究证实，在传统输精管结扎术后长达1年的时间里，人类和豚鼠均未产生对精子的细胞介导免疫。
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