分离细胞核对磷脂囊泡的反应：染色质对脱氧核糖核酸酶I和微球菌核酸酶敏感性的分析
Response of isolated nuclei to phospholipid vesicles: analysis of chromatin sensitivity to DNase I and micrococcal nuclease.
作者信息
Cocco L, Gilmour R S, Papa S, Capitani S, Manzoli F A
出版信息
Cell Biol Int Rep. 1984 Jan;8(1):55-63. doi: 10.1016/0309-1651(84)90182-6.
Phosphatidylserine (PS) and phosphatidylcholine (PC) multilamellar vesicles (MLV) affect chromatin structure as analysed by DNase I sensitivity. The kinetics of DNA solubilisation during the digestion of nuclei indicates that phosphatidylserine causes an increase in DNase accessibility while phosphatidylcholine slightly reduces this accessibility. The effect of phosphatidylserine has also been analysed by means of isokinetic sucrose gradients and agarose gel electrophoresis of nuclear DNA solubilised by micrococcal nuclease. This analysis indicates that phosphatidylserine induces a very rapid production of mononucleosome subunits as compared with untreated nuclei.
通过脱氧核糖核酸酶I敏感性分析可知，磷脂酰丝氨酸（PS）和磷脂酰胆碱（PC）多层囊泡（MLV）会影响染色质结构。细胞核消化过程中DNA溶解的动力学表明，磷脂酰丝氨酸会使脱氧核糖核酸酶的可及性增加，而磷脂酰胆碱会略微降低这种可及性。磷脂酰丝氨酸的作用也通过等速蔗糖梯度和微球菌核酸酶溶解的核DNA的琼脂糖凝胶电泳进行了分析。该分析表明，与未处理的细胞核相比，磷脂酰丝氨酸会诱导单核小体亚基非常快速地产生。
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