Kinnaird J H, Fincham J R
Gene. 1983 Dec;26(2-3):253-60. doi: 10.1016/0378-1119(83)90195-6.
The complete nucleotide sequence of a 2.7-kb genomic fragment, containing the Neurospora crassa am [NADP-specific glutamate dehydrogenase (GDH)] gene, has been determined. The transcription initiation and polyadenylation sites have been defined by S1 mapping. There are at least four initiation sites between 35 and 60 bases downstream of a TATAAA sequence. The single polyadenylation site is immediately downstream of a six-nucleotide sequence which is present in the corresponding position in the N. crassa gene (pl) coding for the imported proteolipid component of mitochondrial ATP synthase [Viebrock et al. Embo J. 1 (1982) 565-571] the initiation sites also show some sequence similarity between am and pl. There are two introns, of 66 and 61 bases respectively; they interrupt sequences that are conserved (highly so in the case of the second intron) between Neurospora and Escherichia coli. As well as showing typical consensus splicing sequences, the two introns show some striking internal similarities to each other. The am gene resembles the pl gene in its strongly selective use of codons (pyrimidines preferred to purines and guanine to adenine in third positions). The coding sequence here reported requires four corrections to be made in the previously published amino acid sequence of Neurospora GDH.
已测定了一个2.7kb基因组片段的完整核苷酸序列，该片段包含粗糙脉孢菌am[NADP特异性谷氨酸脱氢酶（GDH）]基因。转录起始位点和聚腺苷酸化位点已通过S1作图确定。在TATAAA序列下游35至60个碱基之间至少有四个起始位点。单个聚腺苷酸化位点紧接在一个六核苷酸序列的下游，该序列存在于粗糙脉孢菌基因（pl）的相应位置，该基因编码线粒体ATP合酶的导入蛋白脂质成分[维布罗克等人，《欧洲分子生物学组织杂志》1（1982年）565 - 571]，起始位点在am和pl之间也显示出一些序列相似性。有两个内含子，分别为66个碱基和61个碱基；它们中断了粗糙脉孢菌和大肠杆菌之间保守的序列（第二个内含子的情况高度保守）。除了显示典型的共有剪接序列外，这两个内含子彼此之间还显示出一些显著的内部相似性。am基因在密码子的强烈选择性使用上与pl基因相似（嘧啶在第三位比嘌呤更受青睐，鸟嘌呤比腺嘌呤更受青睐）。这里报道的编码序列需要对先前发表的粗糙脉孢菌GDH氨基酸序列进行四处修正。
