Kawanaka M, Hayashi S, Ohtomo H
J Parasitol. 1983 Oct;69(5):857-61.
Newly laid eggs of Schistosoma japonicum were cultured in a serum-free, chemically defined medium, RPMI 1640, which contained 20 amino acids, glutathione, 11 vitamins, and glucose in a balanced salt solution. The requirements for these components in the nutrition of the eggs was investigated by the deletion of single component from the medium. The following 14 amino acids were shown to be essential for the full development of the egg in the medium: L-arginine, L-cystine, glycine, L-histidine, L-isoleucine, L-leucine, L-lysine, L-methionine, L-phenylalanine, L-serine, L-threonine, L-tryptophan, L-tyrosine, and L-valine. Choline chloride was the essential vitamin. The omission of nicotinamide from the medium affected maturation adversely. Glucose was also required by the eggs. Minimal concentration of glucose for maturation of the eggs was 0.02 mM, but concentrations ranging from 0.16 to 20.00 mM gave better results while the concentration of the other elements of the medium were kept constant.
日本血吸虫的新产卵在无血清、化学成分明确的培养基RPMI 1640中培养，该培养基在平衡盐溶液中含有20种氨基酸、谷胱甘肽、11种维生素和葡萄糖。通过从培养基中删除单个成分来研究这些成分在虫卵营养中的需求。结果表明，以下14种氨基酸对培养基中虫卵的充分发育至关重要：L-精氨酸、L-胱氨酸、甘氨酸、L-组氨酸、L-异亮氨酸、L-亮氨酸、L-赖氨酸、L-甲硫氨酸、L-苯丙氨酸、L-丝氨酸、L-苏氨酸、L-色氨酸、L-酪氨酸和L-缬氨酸。氯化胆碱是必需维生素。培养基中缺少烟酰胺会对虫卵成熟产生不利影响。虫卵也需要葡萄糖。虫卵成熟所需的葡萄糖最低浓度为0.02 mM，但当培养基中其他成分的浓度保持恒定时，浓度范围在0.16至20.00 mM时效果更佳。
