Wang S, Zhao W, Zhou M
Hunan Medical University, Changsha.
Zhonghua Yi Xue Za Zhi. 1996 Mar;76(3):218-21.
To study anti-SIEA super-immune sera against the influence on schistosoma japonicum in vitro cultivation of newly laid eggs by adult worms, their hatches and development.
The adult worm pairs of Schistosoma japonicum were collected from test animals, portae vena which infected with the worms for 38-45 days. The worm pairs were injected divided into control group with normal rabbit sera and experimental group with anti-SIEA super-immune sera. The output of newly laid eggs and the embryonic development were observed with immunofluorescence staining.
Female worms cultured in vitro with normal rabbit serum not only discharged eggs already contained in its uterus, but also produced newly formed ones. They however, did not occur in female worms cultured with anti-SIEA super-immune sera and the output of eggs by the latter was much lower than that of the former. There was also a marked decrease in the proportion of developed eggs in the experimental group compared with that in the control group. In addition, immunofluorescence staining of immature eggs and female worms by anti-SIEA super-immune sera showed that a lightened fluorescence was not only seen in the embryonic cells of immature eggs, but also in the vitelaria and in the internal membrane tissue of gut cavity of the female worms.
SIEA was possibly involved in the immunity against egg development and fecundity of schistosoma japonicum.
研究抗日本血吸虫成虫超免疫血清对日本血吸虫成虫体外培养新产卵的孵化及发育的影响。
从感染日本血吸虫38 - 45天的实验动物门静脉采集虫体，将虫体分为两组，一组注射正常兔血清作为对照组，另一组注射抗日本血吸虫成虫超免疫血清作为实验组。采用免疫荧光染色观察新产卵的产出及胚胎发育情况。
用正常兔血清体外培养的雌虫不仅排出子宫内已有的虫卵，还产出新形成的虫卵。然而，用抗日本血吸虫成虫超免疫血清培养的雌虫则未出现这种情况，且后者的产卵量远低于前者。与对照组相比，实验组发育成熟虫卵的比例也显著降低。此外，用抗日本血吸虫成虫超免疫血清对未成熟虫卵和雌虫进行免疫荧光染色显示，不仅在未成熟虫卵的胚胎细胞中观察到荧光减弱，在雌虫的卵黄腺及肠腔内膜组织中也观察到荧光减弱。
日本血吸虫成虫超免疫血清可能参与了对日本血吸虫卵发育及生殖力的免疫作用。
