McPhail L C, Clayton C C, Snyderman R
Science. 1984 May 11;224(4649):622-5. doi: 10.1126/science.6231726.
Arachidonate and other unsaturated long-chain fatty acids were found to activate protein kinase C from human neutrophils. Kinase activation by arachidonate required calcium and was enhanced by diolein but did not require exogenous phosphatidylserine. Submaximal levels of arachidonate also enhanced the affinity of the kinase for calcium during activation by phosphatidylserine. Thus the release of arachidonate, which is triggered in many cell types by ligand-receptor interactions, could play a second messenger role in the regulation of cellular function by activation of protein kinase C.
花生四烯酸和其他不饱和长链脂肪酸可激活人中性粒细胞中的蛋白激酶C。花生四烯酸激活激酶需要钙离子，二油精可增强其激活作用，但不需要外源性磷脂酰丝氨酸。亚最大水平的花生四烯酸也可增强激酶在磷脂酰丝氨酸激活过程中对钙离子的亲和力。因此，在许多细胞类型中由配体-受体相互作用触发的花生四烯酸释放，可能通过激活蛋白激酶C在细胞功能调节中发挥第二信使的作用。
