壁聚合物在再生酵母原生质体上的合成与组装。

Synthesis and assembly of wall polymers on regenerating yeast protoplasts.

作者信息

Sentandreu R, Herrero E, Elorza M V, Rico H, Pastor J

出版信息

Experientia Suppl. 1983;46:187-95. doi: 10.1007/978-3-0348-6776-4_23.

Abstract

Accumulation of chitin and glucan on S. cerevisiae and C. albicans protoplasts begins shortly after resuspension in the regeneration medium, and mannoprotein molecules also appear retained by the regenerating wall after 30-60 minutes in S. cerevisiae or after a longer lag period in C. albicans. Nevertheless, a considerable fraction of the synthesized mannoproteins, which in SDS-acrylamide gels exhibit a different pattern from that of wall manno-proteins of cells, are still released to the growth medium during at least eight hours. De novo synthesis of chitin synthase, but not of glucan synthase, is observed in S. cerevisiae from about 30 minutes after initiation of the regeneration process. The interaction between microfibrils of nascent chitin formed by C. albicans protoplasts is altered by strains as Calcofluor White or Congo Red. In the presence of the former one, no microcrystalline lattice of the polymer is formed and protoplasts do not regenerate correctly.

摘要

几丁质和葡聚糖在酿酒酵母和白色念珠菌原生质体上的积累在重悬于再生培养基后不久就开始了，在酿酒酵母中，30 - 60分钟后，甘露糖蛋白分子似乎也被再生壁保留，而在白色念珠菌中，经过更长的延迟期后也是如此。然而，相当一部分合成的甘露糖蛋白，在SDS - 聚丙烯酰胺凝胶中呈现出与细胞壁甘露糖蛋白不同的模式，在至少八小时内仍会释放到生长培养基中。从再生过程开始约30分钟后，在酿酒酵母中观察到几丁质合酶的从头合成，但未观察到葡聚糖合酶的从头合成。白色念珠菌原生质体形成的新生几丁质微纤维之间的相互作用会被诸如荧光增白剂或刚果红等菌株改变。在前者存在的情况下，聚合物不会形成微晶晶格，原生质体也无法正确再生。