[麦地那龙线虫：使用酶联免疫吸附测定法进行鉴别血清学诊断]

[Filaria medinensis: differential serodiagnosis using ELISA].

作者信息

Perrudet-Badoux A, Boiteau A, Gentilini M

出版信息

Pathol Biol (Paris). 1984 Mar;32(3):182-4.

Abstract

An immunoenzymatic technic ELISA has been used to permit the diagnosis of Dracunculus medinensis . In effect an immunological tool to differentiate helminthiasis, particularly filariosis does not exist yet. For this purpose we have begun to prepare an homologous antigen of a filariosis species ( Dracunculus medinensis ). The antigen extract from the worm has been put in microtiter plaques at the concentration of 25 micrograms/ml with sera diluted 1/1000. The reactions has been performed with anti-G human globulin marked with peroxydase . The results showed that there exist no cross reactivity between Dracunculus medinensis and other helminthiasis even filariosis .

摘要

一种免疫酶技术酶联免疫吸附测定（ELISA）已被用于麦地那龙线虫病的诊断。实际上，目前尚不存在用于区分蠕虫病，特别是丝虫病的免疫学工具。为此，我们已开始制备一种丝虫病物种（麦地那龙线虫）的同源抗原。将从蠕虫中提取的抗原以25微克/毫升的浓度放入微量滴定板中，并与稀释至1/1000的血清混合。反应是用标记有过氧化物酶的抗G人球蛋白进行的。结果表明，麦地那龙线虫与其他蠕虫病甚至丝虫病之间不存在交叉反应。