Scicli A G, Gandolfi R, Carretero O A
Am J Physiol. 1978 Jan;234(1):F36-40. doi: 10.1152/ajprenal.1978.234.1.F36.
The site of formation of kinins in the nephron was determined by stop-flow studies in dogs. Klinin, inulin, sodium, potassium concentrations were measured in the fractions collected during the stop-flow procedures. In addition, in three of the 17 stop-flow experiments, kallikrein activity was also measured. The highest kinin concentration after correction for water reabsorption was found in the fractions that were probably trapped in the distal part of the nephron. Either one or two peak was located either in the fraction overlapping (in one instance) or in the fractions coming prior to the fractions with the highest concentration of potassium. This first peak was present in all but one of the stop-flow experiments and was greater than the second peak. The second peak of kinins was found in 13 of the 17 stop-flow exeriments and was located in the fractions with the lowest sodium concentration. Those fractions with the lowest sodium concentration. Those fractions with the lowest sodium concentration also had the highest kallikrein concentration. No evidence of kinin formation was found in the fractions representing the proximal nephron. We conclude, therefore, that kinins are formed in the distal part of the nephron, with the highest concentration found in the last part of the distal nephron and/or in the renal papilla and pelvis.
通过对狗进行停流实验，确定了肾单位中激肽的形成部位。在停流过程中收集的各部分样本中，测量了激肽、菊粉、钠、钾的浓度。此外，在17次停流实验中的3次实验中，还测量了激肽释放酶的活性。校正水重吸收后，激肽浓度最高的部分可能被困在肾单位的远端。一个或两个峰值要么位于重叠部分（在一个实例中），要么位于钾浓度最高部分之前的部分。除了一次停流实验外，所有实验中都出现了第一个峰值，且第一个峰值大于第二个峰值。在17次停流实验中的13次实验中发现了激肽的第二个峰值，其位于钠浓度最低的部分。那些钠浓度最低的部分激肽释放酶浓度也最高。在代表近端肾单位的部分样本中未发现激肽形成的证据。因此，我们得出结论，激肽在肾单位的远端形成，在远端肾单位的最后部分和/或肾乳头及肾盂中浓度最高。
