Carretero O A, Scicli A G
Fed Proc. 1976 Feb;35(2):194-8.
The distribution of kallikrein in dog kidneys was studied. It was found that kallikrein decreased from the outer to the inner cortex and that the medulla and papilla had very little kallikrein. The site of kallikrein secretion in the nephron was also studied by performing stop-flow techniques in dogs. The highest kallikrein concentration was found in the fractions with the lowest sodium concentration. It was concluded that kallikrein is secreted into the urine at the level of the distal tubule by either the tubule itself or by a structure related to this part of the nephron. In addition, the possible involvement of the kallikrein-kinin system in the regulation of sodium excretion was investigated. Circulating kinins and urinary kallikrein were increased in saline-loaded dogs. Urinary kallikrein also increased in dogs that have "escaped" the sodium-retaining effect of desoxycorticosterone. Experiments in rats with different sodium intake showed a relationship between water and sodium excretion and urinary kallikrein. These data suggest that the kallikrein-kinin system could participate in the regulation of the renal function at the level of the distal tubule or collecting duct.
对狗肾脏中激肽释放酶的分布进行了研究。发现激肽释放酶从肾皮质外层到内层逐渐减少，而髓质和乳头中激肽释放酶含量极少。还通过对狗进行停流技术研究了肾单位中激肽释放酶的分泌部位。在钠浓度最低的部分发现了最高的激肽释放酶浓度。得出的结论是，激肽释放酶是由肾小管本身或与肾单位该部分相关的结构在远曲小管水平分泌到尿液中的。此外，还研究了激肽释放酶 - 激肽系统在钠排泄调节中的可能作用。盐水负荷的狗体内循环激肽和尿激肽释放酶增加。对脱氧皮质酮钠潴留作用产生“逃逸”的狗，其尿激肽释放酶也增加。不同钠摄入量的大鼠实验表明，水和钠排泄与尿激肽释放酶之间存在关联。这些数据表明，激肽释放酶 - 激肽系统可能在远曲小管或集合管水平参与肾功能的调节。
