Uchikawa T, Borle A B
Am J Physiol. 1978 Jan;234(1):R29-33. doi: 10.1152/ajpregu.1978.234.1.R29.
This paper provides mathematical solutions for calculating calcium fluxes and compartments in isolated kidney cells from calcium-45 desaturation curves. In contrast to other available methods, these solutions allow the calculation of kinetic parameters even if isotopic equilibrium has not been reached at the beginning of the desaturation period. To test the validity of the method, calcium-45 desaturation experiments were performed in isolated kidney cells after labeling periods of 30, 60, 90, or 120 min. Even though the calcium-45 desaturation curves differ with different labeling times, the respective values of the calculated parameters are practically identical. The optical labeling period was found to lie between 60 and 120 min. Since this new method does not require initial isotopic steady state, it allows a formal kinetic analysis of tracer desaturation curves even after short periods of labeling.
本文提供了从钙-45去饱和曲线计算分离的肾细胞中钙通量和区室的数学解决方案。与其他现有方法不同，这些解决方案即使在去饱和期开始时未达到同位素平衡，也能计算动力学参数。为了测试该方法的有效性，在标记30、60、90或120分钟后，对分离的肾细胞进行了钙-45去饱和实验。尽管不同标记时间的钙-45去饱和曲线不同，但计算参数的各自值实际上是相同的。发现最佳标记时间在60至120分钟之间。由于这种新方法不需要初始同位素稳态，因此即使在短时间标记后，也能对示踪剂去饱和曲线进行形式动力学分析。
