Uchikawa T, Borle A B
Am J Physiol. 1978 Jan;234(1):R34-8. doi: 10.1152/ajpregu.1978.234.1.R34.
This paper describes a method to measure calcium fluxes and calcium exchangeable pools in tissue slices by continuous perifusion in flow-through chambers. 45Ca desaturation from rat kidney slices can be analyzed as in an open three-compartment catenary system. A set of equations is given to calculate all the relevant kinetic parameters from the triple exponential equations which best fit the desaturation curves. The results show that the kinetic parameters obtained in kidney slices by this new method are in the same order of magnitude as those previously observed in cultured monkey kidney cells.
本文描述了一种通过在流通室中连续灌流来测量组织切片中钙通量和可交换钙池的方法。大鼠肾切片中45Ca的去饱和情况可如同在一个开放的三室链状系统中那样进行分析。给出了一组方程，用于从最能拟合去饱和曲线的三重指数方程计算所有相关的动力学参数。结果表明，用这种新方法在肾切片中获得的动力学参数与先前在培养的猴肾细胞中观察到的参数处于同一数量级。
