Braun-Breton C
Ann Microbiol (Paris). 1984 Mar-Apr;135A(2):181-90. doi: 10.1016/s0769-2609(84)80001-0.
Previously described missense mutations in gene lamB, the structural gene for the lambda receptor in Escherichia coli K12, affected only some of the activities of this multifunctional protein. We isolated lamB mutations, some of which could be of the missense type, and which affected all of the activities of the LamB protein. Among 8 of these mutations, 5 affected the stability of the LamB protein and 3 did not markedly decrease the amount of LamB protein in the mutant strains. In these 3 cases, the mutated LamB proteins were recovered with the envelope of the mutants. We briefly discuss the nature of these mutations and their possible effects on LamB protein structure and location.
先前描述的大肠杆菌K12中λ受体的结构基因lamB的错义突变，仅影响这种多功能蛋白的部分活性。我们分离出了lamB突变，其中一些可能是错义类型，并且影响了LamB蛋白的所有活性。在这些突变中的8个里，5个影响了LamB蛋白的稳定性，3个并未显著降低突变菌株中LamB蛋白的量。在这3种情况下，突变的LamB蛋白与突变体的包膜一起被回收。我们简要讨论了这些突变的性质及其对LamB蛋白结构和定位的可能影响。
