Clément J M, Braun-Breton C, Lepouce E, Marchal C, Perrin D, Villarroya H, Hofnung M
Ann Microbiol (Paris). 1982 Jan;133A(1):9-20.
We describe a system for genetic analysis in gene lamb. It consists of a phage which allows mapping, complementation and sequencing studies of lamB mutations and of the sequence of gene lamB. We present results obtained with this system for a set of mutations conferring tight resistant to phage lambda. This leads to a first identification of three residues in the LamB protein which are important for adsorption of phage lambda h+. Residues 151 and 382 are important for reversible adsorption while residues 401 is important for irreversible adsorption. We briefly review other tight mutations affecting gene lamB and discuss some implications of the results on phage receptor interactions, LamB-protein structure and membrane insertion.
我们描述了一种用于基因lamB遗传分析的系统。它由一个噬菌体组成，该噬菌体可用于lamB突变的定位、互补和测序研究以及基因lamB序列的研究。我们展示了使用该系统对一组赋予对噬菌体λ紧密抗性的突变所获得的结果。这首次鉴定出了LamB蛋白中对噬菌体λh+吸附重要的三个残基。残基151和382对可逆吸附很重要，而残基401对不可逆吸附很重要。我们简要回顾了影响基因lamB的其他紧密突变，并讨论了这些结果对噬菌体受体相互作用、LamB蛋白结构和膜插入的一些影响。
