Nikaein A, Stelzer G, Duquesnoy R J, Wallace J H
Immunobiology. 1984 Mar;166(2):190-202. doi: 10.1016/S0171-2985(84)80037-6.
Responder and stimulator cell subpopulations in the autologous mixed lymphocyte reaction (AMLR) were determined with the OK series of monoclonal antibodies. Mitomycin-C-treated, monocyte-enriched cell populations were used as stimulator cells in the AMLR. Treatment of these monocytes with either OKM and/or OKI monoclonal antibodies and complement resulted in a marked loss of ability of these cells to act as stimulators in the AMLR. Removal of OKT3+ and OKT4+ cells diminished the proliferative responses of AMLR cultures. Interaction of T cells with autologous monocytes resulted in generation of cells capable of suppressing both MLR and AMLR cultures. The suppressor activity of these cells was diminished by treatment with OKI , OKT4 or OKT8 monoclonal antibodies. No cytotoxic activity to autologous or allogeneic monocytes was observed. Additional studies showed an increased number of OKT9 + and OKI + as well as OKT8+ T cells in the AMLR responder cell population. This study indicates that cultures of T lymphocytes with autologous monocytes yield T cell subset(s) which suppress MLR and AMLR reactivity.
采用OK系列单克隆抗体测定了自体混合淋巴细胞反应（AMLR）中的反应细胞和刺激细胞亚群。用丝裂霉素-C处理的富含单核细胞的细胞群体用作AMLR中的刺激细胞。用OKM和/或OKI单克隆抗体及补体处理这些单核细胞，导致这些细胞作为AMLR中刺激细胞的能力显著丧失。去除OKT3 +和OKT4 +细胞可减弱AMLR培养物的增殖反应。T细胞与自体单核细胞的相互作用导致产生能够抑制MLR和AMLR培养物的细胞。用OKI、OKT4或OKT8单克隆抗体处理可减弱这些细胞的抑制活性。未观察到对自体或同种异体单核细胞的细胞毒性活性。进一步的研究表明，AMLR反应细胞群体中OKT9 +、OKI +以及OKT8 + T细胞数量增加。本研究表明，T淋巴细胞与自体单核细胞共培养可产生抑制MLR和AMLR反应性的T细胞亚群。
