Simultaneous visualization of acetylcholinesterase activity and acetylcholine receptor clusters at neuromuscular synapses in vivo and in vitro.

Our double labelling method allows the junctional AChE and AChR distributions to be stained in the same preparation. This method which provides good definition of the fine morphology of synaptic structure and is capable of revealing a very weak AChE activity is of a particular value in studies of synaptogenesis.