The ultrastructural ionic fixation technique localizing acetylcholinesterase activity, reveals simultaneously acetylcholine-like cation in the synaptic vesicles of the motor nerve terminals.
作者信息
Tsuji S
出版信息
Histochemistry. 1984;81(1):35-7. doi: 10.1007/BF00495398.
PMID:6469721
Abstract
A new cytochemical technique is proposed for side by side localization of acetylcholine and of acetylcholinesterase activity of motor end-plate at ultrastructural level. The technique is based on the simultaneous "ionic fixation" of vesicular acetylcholine and of histochemical copper thiocholine precipitate with phosphomolybdic acid: the molybdic heteropolyanion forms insoluble salts with these two quaternary ammonium cations, providing in situ "acetylcholine phosphomolybdate" and "copper thiocholine phosphomolybdate". Both of them are osmium resistant; the electron dense precipitates allow for a fine localization of acetylcholine and acetylcholinesterase activity at electron microscopic level.
摘要
相似文献
1
The ultrastructural ionic fixation technique localizing acetylcholinesterase activity, reveals simultaneously acetylcholine-like cation in the synaptic vesicles of the motor nerve terminals.
Histochemistry. 1984;81(1):35-7. doi: 10.1007/BF00495398.
2
Ultracytochemical localization of acetylcholine-like cations in excited motor end-plates by means of ionic fixation.通过离子固定法对兴奋运动终板中乙酰胆碱样阳离子进行超微细胞化学定位。
Histochemistry. 1985;83(3):213-9. doi: 10.1007/BF00953986.
3
Silicotungstic acid for cytochemical localization of water soluble substance(s) of cholinergic motor nerve terminal.
Histochemistry. 1981;73(1):33-7. doi: 10.1007/BF00493130.
4
Molybdic and tungstic heteropolyanions for "ionic fixation" of acetylcholine in cholinergic motor nerve terminals.
Histochemistry. 1983;77(1):51-6. doi: 10.1007/BF00496635.
5
Electron microscopical autoradiography of [3H]choline fixed by phosphomolybdic acid in the motor nerve terminal.
Neurosci Lett. 1984 Mar 23;45(2):151-6. doi: 10.1016/0304-3940(84)90091-0.
6
Ultrastructural localization of acetylcholinesterase activity by means of the electron dense precipitate derived from Koelle's cuprous thiocholine iodide by treatment with phosphomolybdic acid and osmium tetroxide.通过用磷钼酸和四氧化锇处理从科埃尔碘化亚铜硫代胆碱衍生的电子致密沉淀物来进行乙酰胆碱酯酶活性的超微结构定位。
Histochemistry. 1984;80(1):19-21. doi: 10.1007/BF00492766.
7
Structure of muscle fibres and motor end-plates in the intercostal muscles of lizard, Lacerta agilis L.捷蜥蜴（Lacerta agilis L.）肋间肌中肌纤维和运动终板的结构
Z Mikrosk Anat Forsch. 1974;88(5):796-808.
8
Simultaneous visualization of acetylcholinesterase activity and acetylcholine receptor clusters at neuromuscular synapses in vivo and in vitro.体内和体外神经肌肉突触处乙酰胆碱酯酶活性与乙酰胆碱受体簇的同步可视化。
Biol Cell. 1984;50(1):99-102. doi: 10.1111/j.1768-322x.1984.tb00255.x.
9
Histochemical staining of the acetylcholine receptor, acetylcholinesterase, and the axon terminal.乙酰胆碱受体、乙酰胆碱酯酶和轴突终末的组织化学染色。
Muscle Nerve. 1982 Feb;5(2):140-2. doi: 10.1002/mus.880050211.
10
Electron-microscope cytochemistry of acetylcholine-like cation by means of low-temperature "ionic fixation".
Histochemistry. 1984;81(5):453-5. doi: 10.1007/BF00489749.
引用本文的文献
1
Koelle's copper thiocholine method performed with a low-pH phosphate buffer, followed by osmification of the precipitate: revival of two abandoned procedures.用低pH值磷酸盐缓冲液进行科勒硫代胆碱铜法，随后对沉淀物进行锇化处理：两种被废弃方法的复兴。
Histochemistry. 1984;81(2):129-31. doi: 10.1007/BF00490105.
本文引用的文献
1
A histochemical method for localizing cholinesterase activity.一种用于定位胆碱酯酶活性的组织化学方法。
Proc Soc Exp Biol Med. 1949 Apr;70(4):617-22. doi: 10.3181/00379727-70-17013.
2
THE DIFFERENTIATION OF SYNAPTIC AREAS.突触区域的分化
Proc R Soc Lond B Biol Sci. 1963 Nov 19;158:457-80. doi: 10.1098/rspb.1963.0058.
3
Electron microscopical autoradiography of [3H]choline fixed by phosphomolybdic acid in the motor nerve terminal.
Neurosci Lett. 1984 Mar 23;45(2):151-6. doi: 10.1016/0304-3940(84)90091-0.
4
Ultrastructural localization of acetylcholinesterase activity by means of the electron dense precipitate derived from Koelle's cuprous thiocholine iodide by treatment with phosphomolybdic acid and osmium tetroxide.通过用磷钼酸和四氧化锇处理从科埃尔碘化亚铜硫代胆碱衍生的电子致密沉淀物来进行乙酰胆碱酯酶活性的超微结构定位。
Histochemistry. 1984;80(1):19-21. doi: 10.1007/BF00492766.
5
Molybdic and tungstic heteropolyanions for "ionic fixation" of acetylcholine in cholinergic motor nerve terminals.
Histochemistry. 1983;77(1):51-6. doi: 10.1007/BF00496635.
6
Silicotungstic acid for cytochemical localization of water soluble substance(s) of cholinergic motor nerve terminal.
Histochemistry. 1981;73(1):33-7. doi: 10.1007/BF00493130.
7
[The copper salt-thiocholine reaction used in the histochemical detection of cholinesterases].[用于胆碱酯酶组织化学检测的铜盐 - 硫代胆碱反应]
Ann Histochim. 1970 Oct-Dec;15(4):263-71.
8
[On the localization of cholinesterases by means of thiocholine ester iodides].[利用硫代胆碱酯碘化物对胆碱酯酶进行定位]
C R Acad Hebd Seances Acad Sci D. 1968 Aug 12;267(7):801-3.
9
10
On the chemical basis of thiocholine methods for demonstration of acetylcholinesterase activities.基于硫代胆碱法的乙酰胆碱酯酶活性检测的化学基础。
Histochemistry. 1974;42(1):99-110. doi: 10.1007/BF00498482.
Zotero 插件